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Plant and Cell Physiology Advance Access published online on June 27, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp094
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Dynamic bridges - a calponin-domain kinesin from rice links actin filaments and microtubules in both cycling and non-cycling cells

Nicole Frey, Jan Klotz and Peter Nick*

Institute of Botany 1 and Center for Functional Nanostructures (CFN), University of Karlsruhe, Kaiserstrasse 2, D-76131 Karlsruhe, Germany

*Corresponding author: Peter Nick, Institute of Botany 1, University of Karlsruhe, Kaiserstrasse 2, 76131 Karlsruhe, Germany. Phone +49 721 608 2144, Fax +49 721 608 4193, e-mail peter.nick{at}bio.uni-karlsruhe.de


  Abstract

Interaction and cross-talk between microtubules and actin microfilaments is important for cell axis and polarity during plant cell growth and development, but little is known about the molecular components of this interaction. Plant kinesins with calponin-homology domain (KCH) were recently identified and associated with a putative role in microtubule-microfilament cross-linking. KCHs belong to a distinct branch of the minus end-directed kinesin subfamily and so far have only been identified in land plants including the mosses. Here we report the identification of a new KCH from rice (Oryza sativa), OsKCH1, and show that OsKCH1 is associated with cortical microtubules and actin microfilaments in vivo. Furthermore, OsKCH1 is shown to bind to microtubules and actin microfilaments in vitro in a domain dependent way. Additionally, this unique type of kinesin is shown to oligomerize both in vivo and in vitro. These findings are discussed with respect to a general role of KCHs as linkers between actin filaments and microtubules in both cell elongation and division.

Keywords: actin filaments - microtubules - plant kinesins - KCH - rice

(Received April 28, 2009; Accepted June 26, 2009)
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