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Plant and Cell Physiology Advance Access published online on June 29, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp090
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Differential Gene Expression Profiles of the Mitochondrial Respiratory Components in Illuminated Arabidopsis Leaves.

Keisuke Yoshida1 and Ko Noguchi

Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Corresponding author: Dr. Keisuke Yoshida, Present address: Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 226-8503, Japan, Tel: +81-45-924-5234, Fax: +81-45-924-5268, E-mail: yoshida.k.ao{at}m.titech.ac.jp


  Abstract

Plant mitochondria have multiple energy-dissipating components in the respiratory chain. It is known that these components are induced under several stress conditions. Here we examined whether the gene expression pattern and its regulatory mechanism under high-light (HL) conditions are different among the respiratory components in Arabidopsis leaves. The alternative oxidase (AOX) gene expression (AOX1a and AOX1c) and protein amount were elevated after exposure to HL. In addition to AOX, the expression of other respiratory genes, including NDA1, NDB2, NDC1, UCP1, UCP5, COX6b, and CI76, was also HL-induced. NDB2 was co-expressed with AOX1a, but other HL-induced genes showed a distinct expression pattern. Manipulation of photosynthesis or respiration using several chemicals revealed that, while the expression of AOX1a and NDB2 was mainly induced by the inhibition of the respiratory chain, NDA1 expression was affected by photosynthesis-related reactive oxygen species. The expression of AOX1c, NDC1, COX6b, and CI76 was not induced by these manipulations. When plants were exposed to HL under high CO2 environment, the expression of several respiratory genes was more strongly induced, suggesting that modulations of cellular carbon status by elevated photosynthesis are involved in respiratory gene expression. Based on these results, we propose a mechanistic model of respiratory gene expression in illuminated leaves.

Keywords: alternative oxidase - Arabidopsis thaliana - energy-dissipating respiratory components - gene expression - high-light stress

1Present address: Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 226-8503, Japan

(Received April 30, 2009; Accepted June 16, 2009)
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