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Plant and Cell Physiology Advance Access published online on May 22, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp071
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Differential effects of a transgene to confer low phytic acid in caryopses located at different positions in rice panicles

Mio Kuwano1, Fumio Takaiwa2 and Kaoru T. Yoshida1

1Graduate School of Agricultural and Life Sciences, the University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
2National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan.

Corresponding author: Dr. Yoshida, Kaoru T. Graduate School of Agricultural and Life Sciences, the University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan. Tel: 81-3-5841-8086, Fax: 81-3-5841-1306, E-mail: ayosida{at}mail.ecc.u-tokyo.ac.jp


  Abstract

In previous studies, we attempted to reduce phytic acid in rice seeds by silencing the 1D-myo-inositol 3-phosphate synthase gene, RINO1, using an antisense sequence under control of the rice glutelin GluB-1 promoter. The stable transgenic line showed a weak low-phytic-acid phenotype. In this study, we show that position of the caryopsis in the panicle might affect the level of gene silencing through a difference in temporal and spatial expression patterns between RINO1 and GluB-1 promoters, resulting in a large variation in Pi levels and a small increase in Pi in the transgenic seeds.

Keywords: Gene silencing - Glutelin promoter - Myo-inositol 3-phosphate synthase - Oryza sativa L. - Phytic acid - Transgenic plants

(Received April 7, 2009; Accepted May 19, 2009)
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