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Plant and Cell Physiology Advance Access published online on March 24, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp046
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Pathogen-Induced Expressional Loss of Function Is the Key Factor of Race-Specific Bacterial Resistance Conferred by A Recessive R Gene xa13 in Rice

Meng Yuan, Zhaohui Chu*, Xianghua Li, Caiguo Xu and Shiping Wang

National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China

Corresponding author: Prof. Shiping Wang, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; phone: 86-27-8728-3009; fax: 86-27-8728-7092; email: swang@mail.hzau.edu.cn


  Abstract

The fully recessive disease resistance (R) gene xa13, which mediates race-specific resistance to Xanthomonas oryzae pv. oryzae (Xoo), encodes a plasma membrane protein that differs by one amino acid from that encoded by its dominant (susceptible) allele Xa13. The molecular mechanism of xa13-mediated resistance is largely unknown. Here we show that, compared with its dominant allele, expressional non-reaction of xa13 to Xoo infection, not its protein composition, is the key factor for xa13-mediated resistance. We used the promoter (PXa13) of the dominant Xa13, which was induced by only the incompatible Xoo strain for xa13, to regulate xa13 and xa13Leu49 (a natural recessive allele of xa13) in rice line IRBB13 carrying xa13. The transgenic plants showed the same level of susceptibility and bacterial growth rate as those of the rice line carrying dominant Xa13, accompanied by the induced accumulation of xa13 or xa13Leu49 proteins. Constitutive expression of dominant XA13 or different xa13 proteins (xa13, xa13Leu49, xa13Ala85 or xa13Val184) in IRBB13 had no effect on Xoo infection in the transgenic plants. These results suggest that race-specific pathogen-induced Xa13 expression is critical for infection. Thus, xa13 stands out from other R genes in that its functions in disease resistance are due to only the loss of pathogen-induced transcriptional motivation caused by natural selection.

Keywords: bacterial blight - expression - Oryza sativa - Xanthomonas oryzae

*Current address: College of Plant Protection, Shandong Agricultural University, Taian, China

(Received February 5, 2009; Accepted March 19, 2009)
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