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Plant and Cell Physiology Advance Access published online on March 12, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp042
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Visualization of Plastids in Pollen Grains: Involvement of FtsZ1 in Pollen Plastid Division

Lay Yin Tang1, Noriko Nagata2, Ryo Matsushima1, Yuling Chen3,4, Yasushi Yoshioka3 and Wataru Sakamoto1

1 Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan
2 Department of Chemical Biological Sciences, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan
3 Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan

Corresponding author: Prof. Wataru Sakamoto. Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan. Telephone number: +81-86-434-1206, Fax number: +81-86-434-1206, Email address: saka{at}rib.okayama-u.ac.jp


  Abstract

Visualizing organelles in living cells is a powerful method to analyze their intrinsic mechanisms. Easy observation of chlorophyll facilitates to study the underlying mechanisms in chloroplasts, but not in other plastid types. Here, we constructed a transgenic plant enabling visualization of plastids in pollen grains. Combination of plastid-targeted fluorescent protein with pollen-specific promoter allowed us to observe the precise number, size and morphologies of plastids in pollen grains of wild type and ftsZ1 mutant whose responsible gene plays a central role in chloroplast division. The transgenic material presented in this work is useful for studying division mechanism of pollen plastids.

Keywords: FtsZ1 - Fluorescent protein - Male gametophyte - Plastid division - Pollen

4 Current address: College of Life Science, Hebei Normal University, Shijiazhuang 050016, China

(Received January 29, 2009; Accepted March 4, 2009)
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