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Plant and Cell Physiology Advance Access published online on January 16, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp009
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Mitogen-activated protein kinase cascade in the signaling for polyamine biosynthesis in tobacco

Eun-Kyoung Jang1, Kwang-Hyun Min1, Su-Hyun Kim1, Seung-Hee Nam2, Shuqun Zhang3, Young Cheol Kim1, Baik Ho Cho1 and Kwang-Yeol Yang1,*

1Agricultural Plant Stress Research Center, Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea
2Jeonnam Agricultural Research & Extension Services, Jeonnam, Naju, 520-715, Republic of Korea
3Department of Biochemistry and Bond Life Sciences Center, University of Missouri, Columbia, Missouri, 65211, USA

*Corresponding author: Kwang-Yeol Yang, Agricultural Plant Stress Research Center, Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea Telephone: 82-62-530-2076 FAX: 82-62-530-0207 Email: kyyang{at}chonnam.ac.kr


  Abstract

Expression of NtNEK2DD, a constitutively active mutant of NtMEK2, activates endogenous SIPK and WIPK and leads to several stress/defense responses in tobacco. In this study, we used ACP (annealing control primer)-based differential display RT-PCR to isolate the downstream effectors mediated by the NtMEK2-SIPK/WIPK cascade. The arginine decarboxylase gene (ADC), which is involved in plant putrescine biosynthesis, was one of 9 differentially expressed genes. When compared with NtMEK2KR plants, NtMEK2DD transgenic plants exhibited a significant increase in ADC and ODC transcript levels, as well as in putrescine and its catabolite, GABA, following SIPK/WIPK activation. Taken together, these results suggest that the NtMEK2-SIPK/WIPK cascade is involved in regulating polyamine synthesis, especially putresine synthesis, through transcriptional regulation of the biosynthetic genes in tobacco.

Keywords: Arginine decarboxylase - ACP-based DDRT-PCR - NtMEK2- SIPK/WIPK cascade - Polyamine biosynthesis

(Received October 15, 2008; Accepted January 13, 2009)
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