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Plant and Cell Physiology Advance Access published online on January 15, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp007
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Constitutive and inducible ER bodies of Arabidopsis thaliana accumulate distinct ß-glucosidases

Kimi Ogasawara1,2, Kenji Yamada1,2, John T. Christeller1,3, Maki Kondo2, Noriyuki Hatugai4,5, Ikuko Hara-Nishimura4 and Mikio Nishimura1,2

1Department of Cell Biology, National Institute for Basic Biology, Okazaki, Aichi, 444-8585, Japan
2School of Life Science, Graduate University for Advanced Studies, Okazaki 444-8585, Japan
3Bioprotection Group, HortResearch, Private Bag 11030, Palmerston North 4442, New Zealand
4Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
5PREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan

Corresponding author: Prof. Mikio Nisimura Department of Cell Biology National Institute for Basic Biology Okazaki 444-8585, Japan Tel; +81-564-55-7500 Fax +81-564-55-7505 E-mail; mikosome{at}nibb.ac.jp


  Abstract

The endoplasmic reticulum (ER) body is an ER-related organelle that accumulates high levels of PYK10, a ß-glucosidase with an ER-retention signal. Constitutive ER bodies are present in the epidermal cells of cotyledons, hypocotyls and roots of young Arabidopsis seedlings, but absent in rosette leaves. When leaves are wounded, ER bodies are induced around the wounding site of the leaves (inducible ER bodies). To clarify the functional differences between these two ER bodies, we compared constitutive ER bodies with inducible ER bodies in wounded cotyledons of Arabidopsis seedlings. We found that the number of ER bodies increased both in cotyledons wounded directly (locally-wounded cotyledons) and in unwounded cotyledons exposed to the systemic wound response (systemically-wounded cotyledons). Quantitative RT-PCR and immunoblot analyses revealed that BGLU18, encoding another ß-glucosidase with an ER-retention signal, was induced at the site of wounding, whereas PYK10 was not. Immuno-cytochemical analysis showed that BGLU18 protein was exclusively localized in ER bodies formed directly at the wounding site on cotyledons. ER bodies were not induced in locally- and systemically-wounded cotyledons of the bglu18 knockout mutant. These results indicate that constitutive and inducible ER bodies accumulate different sets of ß-glucosidases and may have distinct functions in defense responses.

Keywords: BGLU18 - ß-glucosidase - PYK10 - ER body - Arabidopsis thaliana - wounding

(Received November 7, 2008; Accepted January 7, 2009)
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