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Plant and Cell Physiology Advance Access published online on January 7, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp002
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Proteomic analysis of salt-responsive proteins in mangrove plant, Bruguiera gymnorhiza

Yuichi Tada and Takaaki Kashimura

School of bioscience and biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan

Corresponding author: Yuichi Tada, School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan E-mail: tadayui{at}bs.teu.ac.jp Telephone: +81 426 37 534 Fax: +81 426 37 5346


  Abstract

To identify key proteins in the regulation of salt tolerance in mangrove plant Bruguiera gymnorhiza, proteome analysis of samples grown under conditions of salt stress was performed. Comparative two-dimensional electrophoresis revealed that two, three and one proteins were differentially expressed in main root, lateral root and leaf, respectively, in response to salt stress. Among these, three proteins were identified by internal peptide sequence analysis: fructose-1,6-bisphosphate aldolase (FBP aldolase) in main root, osmotin in lateral root, and a novel protein in main root. These results suggest that FBP aldolase and osmotin play roles in salt-tolerance mechanisms common to both glycophytes and mangrove plants. Osmotin was abundant at early time points following salt treatment and seems to play a role in initial osmotic adaptation in lateral roots of B. gymnorhiza under salt stress, but does not contribute towards adaptation to prolonged or continuous exposure to salt stress. Amounts of these proteins were not correlated to those of the respective mRNAs, as determined by microarray analysis. A novel salt-responsive protein, not previously detected by EST analysis or transcriptome analysis, was also identified in this proteomic approach, and may provide insight into the salt-tolerance mechanism of the mangrove plant. This is the first report of proteome analysis with detailed analysis of main and lateral roots of mangrove plants under salt stress conditions.

Keywords: Bruguiera gymnorhiza - mangrove - proteome - salt tolerance

(Received November 17, 2008; Accepted January 4, 2009)
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