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Plant and Cell Physiology Advance Access published online on December 19, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn197
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Differential Expression Control and Polarized Distribution of Plasma Membrane-Resident SYP1 SNAREs in Arabidopsis thaliana

Kazuhiko Enami1, Mie Ichikawa1, Tomohiro Uemura2, Natsumaro Kutsuna3,4, Seiichiro Hasezawa3,4, Tsuyoshi Nakagawa5, Akihiko Nakano2,6 and Masa H. Sato1

1 Laboratory of Cellular Dynamics, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5, Shimogamo-nakaragi-cho, Sakyo-ku, Kyoto 606-8522, Japan.
2 Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
3 Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.
4 Institute for Bioinformatics Research and Development (BIRD), Japan Science and Technology Agency (JST)
5 Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue 690-8504, Japan.
6 Molecular Membrane Biology Laboratory, RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan

Corresponding author: Masa H. Sato, Laboratory of Cellular Dynamics, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5, Shimogamo-nakaragi-cho, Sakyo-ku, Kyoto 606-8522 TEL/FAX: +81-75-703-5448 E-mail: mhsato{at}kpu.ac.jp


  Abstract

Membrane trafficking to the plasma membrane (PM) is a highly organized process which enables plant cells to build up their bodies. SNARE genes, which encode the proteins involved in membrane trafficking, are much more abundant in the Arabidopsis genome than that any other eukaryote. We have previously shown that a large number of SNARE molecules in the Arabidopsis cell are localized predominantly on the PM. In the present study, in order to elucidate the physiological function of each PM-localized SNARE, we analyzed the spatiotemporal expression profiling of 9 SYP1s that are resident in the PM of Arabidopsis, and used the information thus acquired to generate transgenic Arabidopsis plants expressing GFP-fused Qa-SNAREs under control of their authentic promoters. Among the 9 SYP1s, only SYP132 is expressed ubiquitously in all tissues throughout plant development. The expression patterns of the other SYP1s, by contrast, are tissue-specific, and all different from one another. A particularly noteworthy example is SYP123, which is predominantly expressed in root-hair cells during root development, and shows a focal accumulation pattern at the tip-region of root hairs. These results suggest that SYP132 is involved in constitutive membrane trafficking to the PM throughout plant development, while the other SYP1s are involved in membrane trafficking events such as root formation or tip-growth of root hair with some redundancy.

Keywords: Arabidopsis - Membrane traffic - Plasma membrane - SNARE - Tip growth

(Received September 28, 2008; Accepted December 15, 2008)
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