Skip Navigation



Plant and Cell Physiology Advance Access published online on December 11, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn194
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow Supplementary Data
Right arrow All Versions of this Article:
50/1/67    most recent
pcn194v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Okamoto, S.
Right arrow Articles by Kawaguchi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okamoto, S.
Right arrow Articles by Kawaguchi, M.
Agricola
Right arrow Articles by Okamoto, S.
Right arrow Articles by Kawaguchi, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Nod factor, nitrate-induced CLE genes that drive systemic regulation of nodulation

Satoru Okamoto1, Erika Ohnishi1, Shusei Sato2, Hirokazu Takahashi3, Mikio Nakazono3, Satoshi Tabata2 and Masayoshi Kawaguchi1,4

1 Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan
2 Kazusa DNA Research Institute, 2-6-7 Kazusa-kaatari, Kisarazu, Chiba 292-0818, Japan
3 Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113–8657, Japan
4 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0112, Japan

Corresponding author: Masayoshi Kawaguchi Address: The University of Tokyo 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan Phone: +81-3-5841-4458, Fax: +81-81-3-5841-4458 E-mail: masayosi{at}biol.s.u-tokyo.ac.jp


  Abstract

Host legumes control root nodule numbers by sensing external and internal cues. A major external cue is soil nitrate, whereas a feedback regulatory system in which earlier formed nodules suppress further nodulation through shoot-root communication is an important internal cue. The latter is known as autoregulation of nodulation (AUT), and is believed to consist of two long-distance signals: a root-derived signal that is generated in infected roots and transmitted to the shoot; and a shoot-derived signal that systemically inhibits nodulation. In Lotus japonicus, the leucine-rich repeat receptor-like kinase, HYPERNODULATION ABERRANT ROOT FORMATION 1 (HAR1), mediates AUT and nitrate inhibition of nodulation, and is hypothesized to recognize the root-derived signal. Here we identify L. japonicus CLE-Root Signal 1 (LjCLE-RS1) and LjCLE-RS2 as strong candidates for the root-derived signal. A hairy root transformation study shows that overexpressing LjCLE-RS1 and -RS2 inhibits nodulation systemically and furthermore, that the systemic suppression depends on HAR1. Moreover, LjCLE- RS2 expression is strongly up-regulated in roots by nitrate addition. Based on these findings, we propose a simple model for AUT and nitrate inhibition of nodulation mediated by LjCLE-RS1, -RS2 peptides and the HAR1 receptor-like kinase.

Keywords: CLE genes - Lotus japonicus - nitrate - Nod factor signalling - nodulation - systemic regulation

The nucleotide sequences reported in this paper have been submitted to DDBJ under accession numbers: LjCLE-RS1, AP010912 [GenBank] ; LjCLE-RS2, AP010911 [GenBank] ; LjCLE3, DF093345 [GenBank] ; LjCLE4, DF093252 [GenBank] ; LjCLE5, AP009981 [GenBank] ; LjCLE6, DF093444 [GenBank] ; LjCLE7, DF093291 [GenBank] ; LjCLE8, DF093487 [GenBank] ; LjCLE9, DF093494 [GenBank] ; LjCLE10, DF093195 [GenBank] ; LjCLE11, DF093446 [GenBank] ; LjCLE12, DF093446 [GenBank] ; LjCLE13, BABK01020001; LjCLE14, BABK01015111; LjCLE15, BABK01005343; LjCLE16, BABK01007606; LjCLE17, DF093443 [GenBank] ; LjCLE18, BABK01015679; LjCLE19, DF093363 [GenBank] ; LjCLE20, DF093363 [GenBank] ; LjCLE21, AP009928 [GenBank] ; LjCLE22, BABK01008074; LjCLE23, BABK01009817; LjCLE24, AP010913 [GenBank] ; LjCLE25, DF093246 [GenBank] ; LjCLE26, DF093353 [GenBank] ; LjCLE27, BABK01010624; LjCLE28, AP010217 [GenBank] ; LjCLE29, BABK01007274; LjCLE30, BABK01023262; LjCLE31, BABK01039053; LjCLE32, DF093533 [GenBank] ; LjCLE33, BABK01016033; LjCLE34, DF093335 [GenBank] ; LjCLE35, DF093323 [GenBank] ; LjCLE36, BABK01068922; LjCLE37, BABK01043991; LjCLE38, BABK01004370; LjCLV3, AP009713.

(Received December 1, 2008; Accepted December 8, 2008)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
B. D. Pant, M. Musialak-Lange, P. Nuc, P. May, A. Buhtz, J. Kehr, D. Walther, and W.-R. Scheible
Identification of Nutrient-Responsive Arabidopsis and Rapeseed MicroRNAs by Comprehensive Real-Time Polymerase Chain Reaction Profiling and Small RNA Sequencing
Plant Physiology, July 1, 2009; 150(3): 1541 - 1555.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.