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Plant and Cell Physiology Advance Access published online on December 26, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn188
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Antisense inhibition of the PsbX protein affects PSII integrity in higher plant Arabidopsis thaliana.

José G. García-Cerdán1, Dmitry Sveshnikov1,4, David Dewez3, Stefan Jansson2, Christiane Funk1,2 and Wolfgang P. Schröder1,2,*

1Dept of Chemistry, Umeå University, SE-901 87 Umeå, Sweden
2Umeå Plant Science Centre (UPSC), Umeå University, SE-901 87 Umeå, Sweden;
3Dept of Plant and Microbial Biology, University of California, Berkeley, California, 94720-3102, USA;
4Potato Research Centre, Agriculture and Agri-Food Canada, PO Box 20280, Fredericton, NB, E3B 4Z7, Canada.

Corresponding author: Wolfgang P. Schröder, Dept of Chemistry, Umeå University, SE-901 87 Umeå, Sweden. E-mail: Wolfgang.Schroder{at}chem.umu.se


  Abstract

Photosystem II, the oxygen-evolving complex of photosynthetic organisms, contains an intriguingly large number of low molecular weight proteins. PsbX, one of these proteins, is ubiquitous in Photosystem II complexes of cyanobacteria and plants. In previous studies, deletion of the PsbX protein in cyanobacteria has not resulted in clear phenotypic changes. Here we report the construction of an anti-sense (AS-PsbX) line in Arabidopsis thaliana, with less than 10% of wild type PsbX levels. AS-PsbX plants are capable of photoautotrophic growth, but biochemical, biophysical, and immunological evidence demonstrates that reduction of PsbX contents leads to reduced levels of functional assembled PSII core complexes, while the light harvesting antennae are not affected. In addition, levels of phosphorylation of the core proteins D1, D2 and CP43 are severely reduced in the anti-sense plants, relative to wild type counterparts. We conclude that PsbX is important for accumulation of functional PSII.

Keywords: Photosynthesis - Photosystem II - reaction centre - thylakoid membrane - chloroplast - OJIP - Thermoluminiscence

(Received September 22, 2008; Accepted November 27, 2008)
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