Plant and Cell Physiology Advance Access published online on December 9, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn180
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Tissue-specific GAL4 expression patterns as a resource enabling targeted gene expression, cell type specific transcript profiling and gene function characterization in the Arabidopsis vascular system
1Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada.
2persent address: Department of Biology, University of Pennsylvania, 415 South Univerity Avenue, Philadelphia, PA, 19104-6018, USA.
3present address: Department of Plant Agriculture, University of Guelph, 50 Stone Road, Guelph, ON, Canada, N1G 2W1.
4present address: Canadian Institute for Advanced Research, Department of Botany, University of British Columbia, 3529-6270 University Boulevard, Vancouver, Canada BC V6T 1Z4.
Corresponding author: e-mail: thomas.berleth{at}utoronto.ca
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Abstract |
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Cell type specific fluorescent gene expression markers are a prerequisite for various strategies in functional genomics and developmental biology. To increase the resolution of vascular tissue analysis and to identify more genes expressed in the vasculature, we searched for expression in vascular tissues within a new collection of transactivated enhancer trap lines. Among thirty-three lines with vascular expression, we identified five lines with expression profiles marking procambial or procambium associated cell states. In stem cross sections we identified one line with phloem- and four with xylem-specific expression as well as nine lines with expression in both phloem and xylem and two with cambial expression. For all lines we also report the expression patterns in different organs and developmental stages. Special features of expression patterns include a line with auxin dependent expression domains. We determined the flanking sequences of twenty-one enhancer-trap insertions, sixteen of which are found in, or in close proximity to annotated genes and thus may reflect the expression patterns of natural promoters. Finally, we analyzed the loss-of-function phenotypes of fourteen putatively affected genes. Remarkably, mutations in a gene encoding a putative F-box protein were associated with an auxin-hypersensitive hypocotyl elongation response. Our compendium provides a diverse selection of markers for different vascular cell states, which can be used for targeted gene expression, cell type specific transcript profiling and gene function assignment in the plant vascular system.
Keywords: Arabidopsis - enhancer trap - GAL4 transactivation - phloem markers - procambium expression - xylem markers
(Received October 26, 2008; Accepted November 19, 2008)
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