Skip Navigation



Plant and Cell Physiology Advance Access published online on November 18, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn173
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrowOA All Versions of this Article:
50/1/78    most recent
pcn173v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Bargmann, B. O. R.
Right arrow Articles by Munnik, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bargmann, B. O. R.
Right arrow Articles by Munnik, T.
Agricola
Right arrow Articles by Bargmann, B. O. R.
Right arrow Articles by Munnik, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

MULTIPLE PLDS REQUIRED FOR HIGH SALINITY AND WATER-DEFICIT TOLERANCE IN PLANTS

Bastiaan O. R. Bargmann1,*, Ana M. Laxalt1,*, Bas ter Riet1,*, Bas van Schooten1, Emmanuelle Merquiol2, Christa Testerink1, Michel A. Haring1, Dorothea Bartels3 and Teun Munnik1,{ddagger}

1Section Plant Physiology, Swammerdam Institute for Life Sciences (SILS), Universiteit van Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands
2Dept. of Ecology and Physiology of Plants, Vrije Universiteit Amsterdam, Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
3Universität Bonn, Molekulare Physiologie und Biotechnologie der Pflanzen, Kirschallee 1, D-53115 Bonn, Germany

{ddagger}corresponding author: Prof. Teun Munnik, t.munnik{at}uva.nl; fax: +31-20-5257934


  Abstract

High salinity and drought have received much attention because they severely affect crop production worldwide. Analysis and comprehension of the plant's response to excessive salt and dehydration will aid in the development of stress tolerant crop varieties. Signal transduction lies at the basis of the response to these stresses and numerous signaling pathways have been implicated. Here, we provide further evidence for the involvement of phospholipase D (PLD) in the plant's response to high salinity and dehydration. A tomato (Lycopersicon esculentum) {alpha}-class PLD, LePLD{alpha}1, is transcriptionally upregulated and activated in cell-suspension cultures treated with salt. Gene-silencing revealed that this PLD is indeed involved in the salt-induced phosphatidic acid production, but not exclusively. Genetically modified tomato plants with reduced LePLD{alpha}1 protein levels did not reveal altered salt tolerance. In Arabidopsis (Arabidopsis thaliana), both AtPLD{alpha}1 and AtPLD{delta} were found to be activated in response to salt stress. Moreover, pld{alpha}1 and pld{delta} single- as well as double knock-out mutants exhibited enhanced sensitivity to high salinity stress in a plate assay. Furthermore, we show that both PLDs are activated upon dehydration and the knock-out mutants are hypersensitive to hyperosmotic stress, displaying strongly reduced growth.

*present addresses: BORB, New York University, Dept. of Biology, 100 Washington Square East, 1009 Silver Building, New York, NY 10003, USA; BtR, The Netherlands Cancer Institute, Plesmalaan 121, NL-1066 CX Amsterdam, The Netherlands; AML, Instituto de Investigaciones Biológicas, Facultad de Ciencas Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina

(Received October 3, 2008; Accepted November 10, 2008)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R. Jost, O. Berkowitz, J. Shaw, and J. Masle
Biochemical Characterization of Two Wheat Phosphoethanolamine N-Methyltransferase Isoforms with Different Sensitivities to Inhibition by Phosphatidic Acid
J. Biol. Chem., November 13, 2009; 284(46): 31962 - 31971.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Darwish, C. Testerink, M. Khalil, O. El-Shihy, and T. Munnik
Phospholipid Signaling Responses in Salt-Stressed Rice Leaves
Plant Cell Physiol., May 1, 2009; 50(5): 986 - 997.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
O. Krinke, M. Flemr, C. Vergnolle, S. Collin, J.-P. Renou, L. Taconnat, A. Yu, L. Burketova, O. Valentova, A. Zachowski, et al.
Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions
Plant Physiology, May 1, 2009; 150(1): 424 - 436.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.