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Plant and Cell Physiology Advance Access published online on November 5, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn170
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

IMAGING OF NPQ and ROS FORMATION IN TOBACCO LEAVES: HEAT INACTIVATION OF THE WATER-WATER CYCLE PREVENTS DOWN-REGULATION OF PS II.

Éva Hideg1, Péter B. Kós1 and Ulrich Schreiber2

1Institute of Plant Biology, Biological Research Center, Szeged, 6701 Hungary
2Julius-von-Sachs Institut für Biowissenschaften, Universität Würzburg, 97070 Germany

Corresponding author: Dr Éva Hideg, Mailing address: P.O.Box 521. H-6701 Szeged, Hungary, Phone: +36 62 599 711, Fax: +36 62 433 434, e-mail: ehideg{at}brc.hu


  Abstract

Non-photochemical chlorophyll fluorescence quenching (NPQ) plays a major role in the protection of the photosynthetic apparatus against damage by excess light, which is closely linked with the production of reactive oxygen species (ROS). The effect of short heat-treatment on NPQ and ROS production was studied with detached tobacco leaves by fluorescence imaging of chlorophyll and of the ROS sensor dye HO-1889NH. NPQ was more than 3-fold stimulated by 3 min pre-treatment at 44°C, in parallel with suppression of CO2 uptake, while no ROS formation could be detected. In contrast, after 3 min pre-treatment at 46°C, NPQ was suppressed and ROS formation was indicated by quenching of HO-1889NH fluorescence. After 3 min pre-treatment at 46°C and above, partial inactivation of the ascorbate peroxidase and light-driven accumulation of H2O2 were also observed. These data are discussed as evidence for a decisive role of the Mehler-Ascorbate-peroxidase or water-water cycle in the formation of the NPQ that reflects down-regulation of Photosystem II.

Keywords: Fluorescence imaging - Heat stress–reactive oxygen species (ROS) - Mehler-Ascorbate-Peroxidase (MAP) cycle - PAM fluorometry - Photosynthetic electron transport

(Received September 5, 2008; Accepted October 31, 2008)
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