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Plant and Cell Physiology Advance Access published online on November 17, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn168
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Highly boron deficiency tolerant plants generated by enhanced expression of NIP5;1, a boric acid channel

Yuichi Kato1,2, Kyoko Miwa2, Junpei Takano2, Motoko Wada1 and Toru Fujiwara2,3,*

1 Graduate school of Agricultural and Life sciences, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
2 Biotechnology Research Center, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
3 SORST, JST

*Corresponding author: Dr. Toru Fujiwara, Biotechnology Research Center, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan, Tel: +81-3-5841-2407; Fax +81-3-5841-2408, E-mail: atorufu{at}mail.ecc.u-tokyo.ac.jp


  Abstract

Boron (B) is an essential element for plants and B deficiency is a worldwide agricultural problem. In B deficient areas, B is often supplied as fertilizer but excess B can be toxic to both plants and animals. Generation of B-deficiency tolerant plants could reduce B fertilizer use. Improved fertility under B-limiting conditions in Arabidopsis thaliana by overexpression of BOR1, a B transporter, has been reported, but the root growth was not improved by the BOR1 overexpression. In this paper, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B limiting conditions in A. thaliana. An NIP5;1 activation tag line, which has a T-DNA insertion with enhancer sequences near the NIP5;1 gene, showed improved root elongation under B limitation. We generated a construct which mimics the tagline: the cauliflower mosaic virus 35S RNA promoter was inserted at the 1357 bp upstream of NIP5;1 transcription initiation site. Introduction of this construct into nip5;1-1 mutant and BOR1 over-expresser resulted in enhanced expression of NIP5;1 and improved root elongation under low B supply. Furthermore, one of transgenic lines exhibited improved fertility and short-term B uptake. Our results demonstrate successful improvement of B deficiency tolerance and potentials of enhancing expression of a mineral nutrient channel gene to improve growth under nutrient limiting conditions.

Keywords: Arabidopsis thaliana - boron - channel - deficiency tolerance - nodulin 26-like intrinsic proteins - transporter

(Received August 30, 2008; Accepted October 30, 2008)
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