Plant and Cell Physiology Advance Access published online on November 6, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn166
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Characterization of OsPIP2;7, a water channel protein in rice
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, and Graduate School of the Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China
Corresponding author: Wei-Ai Su, 300 Fenglin Road, Shanghai 200032, China, Tel: (86) 21 54924245, Fax: (86) 21 54924015, E-mail: zstressc{at}online.sh.cn
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Abstract |
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Aquaporins are water channel proteins that facilitate water and other small neutral molecules across the biological membranes. There are usually a large number of members in higher plants, which exhibit various physiological functions and are regulated in a time and special mode. We have previously shown that a rice gene, OsPIP2;7, was generally up-regulated in roots but down-regulated in shoots at the early stage of chilling stress. Here, OsPIP2;7 was cloned and proved to be an aquaporin with high activity in Xenopus oocytes. OsPIP2;7 was localized mainly in mesophyll cells of leaves. In roots it was detected in the vascular tissues, epidermis cells and exodermis cells at the elongation zone, as well as in the epidermis cells, exodermis cells and root hair at the maturation zone. The yeast cells overexpressing OsPIP2;7 showed higher survival rate after freeze/thaw stress. Furthermore, OsPIP2;7 enhanced the plants' transpiration rate and tolerance to low temperature when overexpressed in rice. These results indicated that OsPIP2;7 was involved in water rapid transport and maintenance of the water balance in cells, ultimately improves the tolerance of the yeast and rice to low temperature stress.
Keywords: in situ hybridization expression - low temperature stress - rice - water channel activity - yeast
*These authors contributed equally to this paper.
(Received August 19, 2008; Accepted October 28, 2008)
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