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Plant and Cell Physiology Advance Access published online on October 9, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn152
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Interplay of RNA Pol IV and ROS1 during postembryonic 5S rDNA chromatin remodelling.

Julien Douet, Bertrand Blanchard1, Claudine Cuvillier and Sylvette Tourmente*

CNRS, UMR 6247 GReD; Clermont Université; INSERM; 24 Avenue des Landais, 63177 Aubière Cedex, France

Corresponding author: Sylvette Tourmente. Address: UMR GReD, CNRS, Université Blaise Pascal, 24 Avenue des Landais, 63177 Aubière Cedex, France, Tel: (33) 4 73 40 74 01, FAX: (33) 4 73 40 77 77, sylvette.tourmente{at}univ-bpclermont.fr


  Abstract

We have investigated the chromatin structure of 5S ribosomal DNA, a heterochromatic pericentromeric tandemly repeated family, at 2, 3, 4 and 5 days post-germination. Our results revealed a large-scale re-organization of 5S rDNA chromatin that occurs during the first days of development. Unexpectedly, there is a decondensation followed by a "re"condensation of 5S rDNA chromatin, to obtain almost mature nuclei 5 days post-germination. The re-organization of 5S rDNA chromatin is accompanied by a rapid and active demethylation of 5S rDNA mediated by the ROS1 demethylase whereas the plant specific RNA Polymerase IV is essential to the 5S chromatin "re"condensation. In conclusion, Pol IV and ROS1 collaborate to unlock the 5S rDNA chromatin inherited from the seed, and establish adult features.

Keywords: 5S rDNA - Arabidopsis thaliana - chromatin - RNA Polymerase IV - ROS1

1 Present address: CERMAV-CNRS, Université Joseph Fourier, 601 rue de la Chimie, BP53 38041 Grenoble Cedex 9, France

(Received September 13, 2008; Accepted October 7, 2008)
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