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Plant and Cell Physiology Advance Access published online on October 14, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn151
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Abscisic acid does not disrupt either the Arabidopsis FCA-FY interaction or its rice counterpart in vitro

Yun Hee Jang, Jeong Hwan Lee and Jeong-Kook Kim*

Plant Signaling Network Research Center, School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

*Corresponding author: Dr. Jeong-Kook Kim. Plant Signaling Network Research Center, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea. e-mail; jkkim{at}korea.ac.kr, Tel: +82-2-3290-3157, Fax: +82-2-927-9028


  Abstract

We examined the effect of (+)-Abscisic acid (ABA) on the in vitro interaction of rice FCA and FY homologues, OsFCA and OsFY. From this analysis, we found no disruption of the OsFCA-OsFY complexes by ABA treatment. This result prompted us to examine the effect of ABA on the FCA-FY interaction. In these experiments, we could not reproduce the inhibitory effect of (+)-ABA on the interaction between FCA and FY. Based on these combined results, we believe the inhibitory effect of (+)-ABA on the FCA-FY interaction should be cautiously reconsidered.

Keywords: Abscisic acid - FCA and FY interaction - OsFCA and OsFY interaction - GST pull-down assay - ABA receptor

(Received September 12, 2008; Accepted October 7, 2008)
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