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Plant and Cell Physiology Advance Access published online on October 11, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn149
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Degradation of Sphingoid Long-chain base 1-Phosphates (LCB-1Ps): Functional Characterization and Expression of AtDPL1 Encoding LCB-1P Lyase Involved in Dehydration Stress Response in Arabidopsis

Masahiro Nishikawa1, Kenta Hosokawa1, Mai Ishiguro1, Hiroki Minamioka1, Kentaro Tamura2, Ikuko Hara-Nishimura2, Yohei Takahashi3, Ken-ichiro Shimazaki3 and Hiroyuki Imai1,4

1 Department of Biology, Graduate School of Natural Science, Konan University, Kobe 658-8501, Japan
2 Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan
3 Department of Biology, Faculty of Science, Kyushu University, Fukuoka 810-8560, Japan

4Corresponding author: Dr. Hiroyuki Imai, Department of Biology, Graduate School of Natural Science, Konan University, Kobe 658-8501 Japan, Tel: 078-435-2513; FAX: 078-435-2539; e-mail: imai{at}konan-u.ac.jp


  Abstract

Sphingoid long-chain bases (LCB) 1-phosphates are degradated by LCB 1-phosphate lyase to C16 fatty aldehydes and phosphoethanolamine. Here, we confirmed that the At1g27980 gene product, AtDPL1, is a functional LCB-1-phosphate lyase. Expression of green fluorescent protein fusion products in suspension-cultured Arabidopsis cells showed that AtDPL1 is located to the endoplasmic reticulum. The rates of fresh weight decreases of dpl1-1 and dpl1-2 mutants were significantly slower than those of the wild-type plants. This ability to limit their transpiration reflected the leaf temperature of the mutant plants higher than that of wild-type plants, suggesting that AtDPL1 plays a role in dehydration stress.

Keywords: dehydration stress - long-chain base - sphingosine-1-phosphate lyase - transpiration

(Received July 16, 2008; Accepted October 6, 2008)
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