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Plant and Cell Physiology Advance Access published online on September 30, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn146
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Two CAP BINDING PROTEINS CBP20 and CBP80 are involved in processing primary microRNAs

Sanghee Kima, Jun-Yi Yanga, Jun Xua, In-Cheol Janga, Michael J. Priggeb and Nam-Hai Chuaa

aLaboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065-6399, USA
bDepartment of Biology, Indiana University, 915 East Third Street, Bloomington, IN 47405

Corresponding author: Prof. Nam-Hai Chua, Tel: +1-212-327-8126; Fax: +1-212-327-8327; E-mail: chua{at}mail.rockefeller.edu; Address: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065-6399, USA


  Abstract

MicroRNAs are 21nt RNAs that regulate many biological processes in plants by mediating translational inhibition or cleavage of target transcripts. Arabidopsis mutants defective in miRNA biogenesis have overlapping and highly pleiotropic phenotypes including serrated leaves and ABA hypersensitivity. Recent evidence indicates that miRNA genes are transcribed by RNA polymerase II. Since Pol II transcripts are capped we hypothesized that CBP (CAP BINDING PROTEIN) 20 and 80 may bind to capped pri-miRNA transcripts and play a role in their processing. Here, we show that cbp20 and cbp80 mutants have reduced miRNA levels and increased pri-miRNA levels. Co-immunoprecipitation experiments revealed that pri-miRNAs 159, 166, 168 and 172 could be associated with CBP20 and CBP80. We found that CBP20 and CBP80 are stabilized by ABA by a post-translational mechanism, and these proteins are needed for ABA induction of miR159 during seed germination. The lack of miR159 accumulation in ABA treated seeds of cbp20/80 mutants leads to increased MYB33 and MYB101 transcript levels and presumably higher levels of these positive regulators result in ABA hypersensitivity. Genetic and molecular analyses show that CBP20 and 80 have overlapping function in the same developmental pathway as SE and HYL1. Our results identify new components in miRNA biogenesis.

Keywords: CAP BINDING PROTEIN - microRNA - primary microRNA - ABA - Post-translational modification

(Received July 11, 2008; Accepted September 22, 2008)
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