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Plant and Cell Physiology Advance Access published online on September 17, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn140
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Effect of PGR5 impairment on photosynthesis and growth in Arabidopsis thaliana

Yuri Nakajima Munekagea, Bernard Genty and Gilles Peltier

CEA Cadarache, DSV, IBEB, SBVME, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Laboratoire d'Ecophysiologie Moléculaire des Plantes, UMR 6191 CNRS/CEA/ Université Aix-Marseille, F-13108 Saint-Paul-lez-Durance, France

Corresponding author: Yuri Nakajima Munekage Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, 630-0192 Japan. Tel: +81 743 72 5568, FAX: +81 743 72 5569, E-mail: munekage{at}bs.naist.jp


  Abstract

PGR5 has been reported as an important factor for the activity of the ferredoxin-dependent cyclic electron transport around photosystem I. To elucidate the role of PGR5 on C3 photosynthesis, we characterize photosynthetic electron transport rate (ETR), CO2 assimilation and growth in the Arabidopsis thaliana pgr5 mutant in various irradiance and CO2 regimes. In low light grown pgr5, CO2 assimilation rate and ETR were similar to the wild-type at low irradiance, but decreased at saturating irradiance under photorespiratory conditions as well as non-photorespiratory conditions. Although non-photochemical quenching of chlorophyll fluorescence (NPQ) was not induced in pgr5 under steady-state photosynthesis, we show that it was induced under dark to light transition at low CO2 concentration. Under low light conditions in air, pgr5 showed the same growth as the wild-type but a significant growth reduction compared to the wild-type above 150 µmol photons m–2 s–1. This growth impairment was largely suppressed under high CO2 concentration. Based on the intercellular CO2 concentration dependency of CO2 assimilation, ETR and P700 oxidation measurements, we conclude that reduction of photosynthesis and growth result from 1) ATP deficiency and 2) inactivation of photosystem I. We discuss these data in relation to the role of PGR5-dependent regulatory mechanisms in tuning the ATP/NADPH ratio and preventing inactivation of photosystem I, especially under conditions of high irradiance or enhanced photorespiration.

Keywords: cyclic electron transport - photosynthesis - CO2 assimilation - photoinhibition - non-photochemical quenching

aPresent address: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, 630-0192 Japan

(Received July 14, 2008; Accepted September 7, 2008)
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