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Plant and Cell Physiology Advance Access first published online on September 6, 2008
This version published online on October 8, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn128
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
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Various spatiotemporal expression profiles of anther-expressed genes in rice

Tokunori Hobo1,*, Keita Suwabe2,*, Koichiro Aya1, Go Suzuki3, Kentaro Yano4, Takeshi Ishimizu5, Masahiro Fujita6, Shunsuke Kikuchi4, Kazuki Hamada4, Masumi Miyano2,7, Tomoaki Fujioka2, Fumi Kaneko2, Tomohiko Kazama2,7, Yoko Mizuta6, Hirokazu Takahashi8, Katsuhiro Shiono8, Mikio Nakazono8, Nobuhiro Tsutsumi8, Yoshiaki Nagamura9, Nori Kurata6, Masao Watanabe2,7,10,** and Makoto Matsuoka1,**

1 Bioscience and Biotechnology Center, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan
2 Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan
3 Division of Natural Science, Osaka Kyoiku University, Kashiwara 582-8582, Japan
4 Faculty of Agriculture, Meiji University, Kawasaki 214-8571, Japan
5 Department of Chemistry, Graduate School of Science, Osaka University, Osaka 560-0043, Japan
6 Plant Genetics Laboratory, National Institute of Genetics, Mishima 411-8540, Japan
7 The 21st Century Center of Excellence Program, Iwate University, Morioka 020-8550, Japan
8 Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
9 Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
10 Faculty of Science, Tohoku University, Sendai 980-8587, Japan

**Corresponding author: Dr. Masao Watanabe, Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University, 2-1-1, Katahira, Aoba-ku, Sendai 980-8577, Japan, Tel: +81-(0) 22-217-5681, Fax: +81-(0) 22-217-5683, E-mail: nabe{at}ige.tohoku.ac.jp. Dr. Makoto Matsuoka, Laboratory of Plant Molecular Breeding, Bioscience and Biotechnology Center, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan, Tel: +81-(0) 52-789-5218, Fax: +81-(0) 52-789-5226, E-mail: makoto{at}nuagr1.agr.nagoya-u.ac.jp


  Abstract

The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using the laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into the 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in the anther development, such as pollen maturation, pollen germination, pollen-tube elongation, and pollen-wall formation.

Keywords: anther - gene ontology - laser microdissection - microarray - Oryza sativa L. - synchronous gene expression

*These authors equally contributed to this work

(Received July 20, 2008; Accepted August 30, 2008)
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