Skip Navigation



Plant and Cell Physiology Advance Access published online on September 4, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn127
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
49/10/1572    most recent
pcn127v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Riewe, D.
Right arrow Articles by Geigenberger, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Riewe, D.
Right arrow Articles by Geigenberger, P.
Agricola
Right arrow Articles by Riewe, D.
Right arrow Articles by Geigenberger, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

A cell wall bound adenosine nucleosidase is involved in the salvage of extracellular ATP in Solanum tuberosum

David Riewe1, Lukasz Grosman1, Alisdair R. Fernie1, Henrik Zauber1, Cornelia Wucke1 and Peter Geigenberger1,2,*

1Max-Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany
2Leibniz-Institute of Vegetable and Ornamental Crops, Theodor-Echtermeyer-Weg 1, 14979 Grossbeeren, Germany

*Corresponding Author: Dr. Peter Geigenberger, Leibniz-Institute of Vegetable and Ornamental Crops, Theodor-Echtermeyer-Weg 1, 14979 Grossbeeren, Germany, Mail: geigenberger{at}igzev.de, Phone: +49-33701-78220, Fax: +49-33701-55391


  Abstract

Extracellular ATP (eATP) has recently been demonstrated to play a crucial role in plant development and growth. To investigate the fate of eATP within the apoplast, we used intact potato (Solanum tuberosum) tuber slices as experimental system enabling access to the apoplast without interference of cytosolic contaminations. (i) Incubation of intact tuber slices with ATP led to the formation of ADP, AMP, adenosine, adenine and ribose, indicating operation of apyrase, 5'-nucleotidase and nucleosidase. (ii) Measurement of apyrase, 5'-nucleotidase and nucleosidase activities in fractionated tuber tissue confirmed apoplastic localization for apyrase and phosphatase in potato and led to the identification of a novel cell-wall bound adenosine nucleosidase activity. (iii) When intact tuber slices were incubated with saturating concentrations of adenosine, the conversion of adenosine into adenine was much higher than adenosine import into the cell suggesting a potential bypass of adenosine import. Consistent with this, import of radio-labeled adenine into tuber slices was inhibited when ATP, ADP or AMP were added to the slices. (iv) In wild type plants, apyrase and adenosine nucleosidase activities were found to be co-regulated, indicating functional linkage of these enzymes in a shared pathway. (v) Moreover, adenosine nucleosidase activity was reduced in transgenic lines with strongly reduced apoplastic apyrase activity. When taken together, these results suggest that a complete ATP-salvage pathway is present in the apoplast of plant cells.

Keywords: extracellular ATP - signaling - apyrase - adenosine nucleosidase - apoplast - cell wall - Solanum tuberosum

(Received July 11, 2008; Accepted August 25, 2008)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
B. Jung, M. Florchinger, H.-H. Kunz, M. Traub, R. Wartenberg, W. Jeblick, H. E. Neuhaus, and T. Mohlmann
Uridine-Ribohydrolase Is a Key Regulator in the Uridine Degradation Pathway of Arabidopsis
PLANT CELL, March 1, 2009; 21(3): 876 - 891.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.