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Plant and Cell Physiology Advance Access published online on August 28, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn124
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray

Keita Suwabe1,*, Go Suzuki2,*, Hirokazu Takahashi3, Katsuhiro Shiono3, Makoto Endo4, Kentaro Yano5, Masahiro Fujita6, Hiromi Masuko1, Hiroshi Saito7, Tomoaki Fujioka1, Fumi Kaneko1, Tomohiko Kazama1,7, Yoko Mizuta6, Makiko Kawagishi-Kobayashi4, Nobuhiro Tsutsumi3, Nori Kurata6, Mikio Nakazono3 and Masao Watanabe1,7,8,**

1 Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan
2 Division of Natural Science, Osaka Kyoiku University, Kashiwara 582-8582, Japan
3 Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
4 Laboratory of Biotechnology, National Institute of Crop Science, Tsukuba 305-8518, Japan
5 Faculty of Agriculture, Meiji University, Kawasaki 214-8571, Japan
6 Plant Genetics Laboratory, National Institute of Genetics, Mishima 411-8540, Japan
7 The 21st Century Center of Excellence Program, Iwate University, Morioka 020-8550, Japan
8 Faculty of Science, Tohoku University, Sendai 980-8578, Japan

**Corresponding author: Dr. Masao Watanabe, Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University, 2-1-1, Katahira, Aoba-ku, Sendai 980-8577, Japan, Tel: +81-(0) 22-217-5681, Fax: +81-(0) 22-217-5683, E-mail: nabe{at}ige.tohoku.ac.jp


  Abstract

In flowering plants, the male gametophyte, pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. Expression profiles of 11 known tapetum specific-genes were consistent with previous reports. Based on their spatial and temporal expression patterns, 140 genes which had been previously defined as anther-specific were further classified as male gametophyte-specific (71 genes, 51%), tapetum-specific (7 genes, 5%), or expressed in both male gametophyte and tapetum (62 genes, 44%). These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum.

Keywords: anther - laser microdissection - microarray - Oryza sativa L. - microspore/pollen - tapetum

* These authors equally contributed to this work

(Received July 20, 2008; Accepted August 15, 2008)
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