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Plant and Cell Physiology Advance Access published online on July 16, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn103
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

A Unique Caleosin in Oil Bodies of Lily Pollen

Pei-Luen Jiang1, Guang-Yuh Jauh2, Co-Shing Wang1 and Jason T.C. Tzen1,3,*

1 Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan
2 Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan
3 Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan

*Corresponding author: Prof. Jason T.C. Tzen. Graduate Institute of Agricultural Biotechnology National Chung-Hsing University Taichung, Taiwan, 40227. Phone: 886-4-22840328, Fax: 886-4-22853527, E mail address: TCTZEN{at}dragon.nchu.edu.tw


  Abstract

In view of the recent isolation of stable oil bodies as well as a unique oleosin from lily pollen, this study aimed to examine if other minor proteins were present in this lipid-storage organelle. Immunological cross-recognition using antibodies against three minor oil-body proteins from sesame suggested that a putative caleosin was specifically detected in the oil-body fraction of pollen extract. A cDNA fragment encoding this putative pollen caleosin, obtained by PCR cloning, was confirmed by immunodetection and MALDI-MS analyses of the recombinant protein over-expressed in Escherichia coli and the native form. Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies.

Keywords: caleosin - lily (Lilium longiflorum Thunb.) - oil bodies - oleosin - pollen

(Received May 15, 2008; Accepted July 13, 2008)
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