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Plant and Cell Physiology Advance Access published online on July 1, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn098
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Live cell imaging reveals plant Aurora kinase has dual roles during mitosis

Daisuke Kurihara, Sachihiro Matsunaga, Susumu Uchiyama and Kiichi Fukui*

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

*Corresponding author: Dr. Kiichi Fukui. E-mail: kfukui{at}bio.eng.osaka-u.ac.jp; Tel: +81-6-6879-7440 / Fax: +81-6-6879-7441


  Abstract

The proper segregation of chromosomes during mitosis is required for accurate distribution of genetic information by two daughter cells. Here, we used live cell imaging of microtubules and kinetochores after an Aurora kinase inhibitor, Hesperadin treatment in tobacco BY-2 cells to analyze the function of plant Aurora kinase during mitosis. Hesperadin treatment induced the delay of CenH3 alignment on the spindle equator. Furthermore, two types of dynamics of lagging CenH3s were observed during chromosome segregation. The findings indicate that the plant Aurora kinase has dual roles; correction of aberrant kinetochore-microtubule attachment and dissociation of cohesin during chromosome alignment and segregation.

Keywords: chromosome segregation - cohesin dissociation - kinetochore-microtubule attachment - lagging chromosome - plant Aurora kinase

(Received April 21, 2008; Accepted June 26, 2008)
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