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Plant and Cell Physiology Advance Access published online on June 17, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn091
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Jasmonate perception regulates jasmonate biosynthesis and JA-Ile metabolism: the case of COI1 in Nicotiana attenuata

Anja Paschold1, Gustavo Bonaventure, Merijn R. Kant and Ian T. Baldwin

Max Planck Institute for Chemical Ecology, Department of Molecular Ecology, Hans-Knöll-Strasse 8, 07745 Jena, Germany.

Corresponding author: Dr. Ian T. Baldwin, Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Strasse 8, 07745 Jena, Germany, Phone: 49-(0)3641-571100, Fax: 49-(0)3641-571102, Email: Baldwin{at}ice.mpg.de


  Abstract

CORONATINE INSENSITIVE 1 (COI1) is a well-known key player in processes downstream of JA biosynthesis: silencing COI1 in Nicotiana attenuata (ir-coi1) makes plants insensitive to jasmonic acid (JA), prevents the up-regulation of JA-mediated defenses and decreases the plant's resistance to herbivores and pathogens. In agreement with previous studies, we observed that regulation of several JA biosynthesis genes elicited by Manduca sexta oral secretions (OS) is COI1-dependent. In response to wounding and application of OS ir-coi1 plants accumulate 75% less JA compared to wild type plants (WT) resembling JA levels found in plants silenced in the key enzyme in JA biosynthesis LIPOXYGENASE 3 (as-lox). However, while OS-elicited as-lox plants also accumulated lower levels of the JA-conjugate JA-isoleucine (JA-Ile) than WT plants, JA-Ile accumulation in ir-coi1 was higher, prolonged and peaked with a delay of 30 minutes. In vivo substrate feeding experiments of N. attenuata demonstrate that the increased and prolonged JA-Ile accumulation pattern in ir-coi1 is not the result of altered substrate availability, i.e. of JA and/or Ile, but is due to a ~6-fold decrease in JA-Ile turnover. These results provide the first evidence for a second, novel regulatory feed-back function of COI1 in enhancing JA-Ile turnover. Hence, in addition to its control over JA biosynthesis, COI1 might fine tune the dynamics of the jasmonate response after induction by herbivore elicitors.

Keywords: Coronatine insensitive1 - jasmonic acid - jasmonic acid- isoleucine - Nicotiana attenuata - Solanum lycopersicum

1Current address: Centre for Plant Molecular Biology (ZMBP), Department of General Genetics, Eberhard-Karls-University, Auf der Morgenstelle 28, 72076 Tübingen, Germany.

(Received April 13, 2008; Accepted June 8, 2008)
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