Skip Navigation



Plant and Cell Physiology Advance Access published online on June 11, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn090
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
49/8/1150    most recent
pcn090v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Lv, S.
Right arrow Articles by Zhang, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lv, S.
Right arrow Articles by Zhang, J.
Agricola
Right arrow Articles by Lv, S.
Right arrow Articles by Zhang, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Overexpression of an H+-PPase gene from Thellungiella halophila in cotton enhances salt tolerance and improves growth and photosynthetic performance

Sulian Lv, Kewei Zhang, Qiang Gao, Lijun Lian, Yingjie Song and Juren Zhang*

School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China

*Corresponding author: Prof. Juren Zhang. School of Life Science, Shandong University, 27 Shanda South Road, Jinan, P. R. China 250100. E-mail: jrzhang{at}sdu.edu.cn, Tel: +86 531 88364350, Fax: +86 531 88564350


  Abstract

Salinity is one of the major environmental factors limiting plant growth and productivity. An H+-PPase gene, TsVP from Thellungiella halophila, was transferred into cotton (Gossypium hirsutum) in sense and antisense orientations under control of the CaMV 35S promoter. Southern and northern blotting analysis showed the sense or antisense TsVP were integrated into the cotton genome and expressed. Transgenic plants overexpressing the vacuolar H+-pyrophosphatase were much more resistant to 150 mM and 250 mM NaCl than the isogenic wild-type plants. In contrast, the plants from the antisense line (L-2), with lower H+-PPase activity, were more sensitive to salinity than the wild type ones. Overexpressing TsVP in cotton improved shoot and root growth and photosynthetic performance. These transgenic plants accumulated more Na+, K+,Ca2+, Cl- and soluble sugars in their root and leaf tissues under salinity conditions compared with the wild type ones. The lower membrane ion leakage and MDA level in these transgenic plants suggest overexpression of H+-PPase causes the accumulation of Na+ and Cl- in vacuoles instead of in the cytoplasm thus reducing their toxic effects. In the other hand, the increased ions and sugars accumulation decreases the solute potential in cells, and facilitates water uptake under salinity, which is an important mechanism for the increased salt tolerance in TsVP-overexpressing cotton.

Keywords: Cotton (Gossypium hirsutum) - H+ - PPase - Photosynthesis - Salt tolerance

(Received March 30, 2008; Accepted June 7, 2008)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol PlantHome page
A. Amtmann
Learning from Evolution: Thellungiella Generates New Knowledge on Essential and Critical Components of Abiotic Stress Tolerance in Plants
Mol Plant, January 1, 2009; 2(1): 3 - 12.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.