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Plant and Cell Physiology Advance Access published online on June 4, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn083
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

NDF6; A thylakoid protein specific to terrestrial plants is essential for activity of chloroplastic NAD(P)H dehydrogenase in Arabidopsis.

Noriko Ishikawa1, Atsushi Takabayashi2, Satoshi Ishida1, Yasushi Hano1, Tsuyoshi Endo1,* and Fumihiko Sato1

1Guraduate School of Biostudies, Kyoto University, Sakyoku,Kyoto, 606-8502, Japan
2Center for Gene Research, Nagoya University, Nagoya, Aichi 464-8602, Japan

*Corresponding Author: Dr. Tsuyoshi Endo, Graduate School of Biostudies, Kyoto University, Sakyoku, Kyoto 606-8502, Japan. tel: +81-(0)75-753-6381, fax: +81-(0)75-753-6398, e-mail: tuendo{at}kais.kyoto-u.ac.jp


  Abstract

NAD(P)H dehydrogenase (NDH) is a homologue of respiratory complex I and mediates one of the two pathways of cyclic electron flow around photosystem I (CEF I). Although 15 ndh subunits have been identified in the chloroplastic and nuclear genomes of higher plants, no electron-accepter subunits have been identified to date. To identify the missing chloroplastic NDH subunits, we undertook an in silico approach based on co-expression analysis. In this report, we characterized the novel gene NDF6 (NDH dependent flow 6; At1g18730) which encodes a protein that is essential for NDH activity. NDF6 has one trans-membrane domain and is localized in the thylakoid membrane fraction. Homologous proteins of NDF6 were identified in the genomes of terrestrial plants; however, no homologues have been found in cyanobacteria, which are thought to be the origin of chloroplasts and have a minimal NDH complex unit. NDF6 is unstable in ndhB-impaired or disrupted mutants of higher plants in which the chloroplastic NDH complex is thought to be degraded. These results suggest that NDF6 is a novel subunit of chloroplastic NDH that was added to terrestrial plants during evolution.

Keywords: NAD(P)H dehydrogenase - cyclic electron flow around photosystem I - co-expression - Arabidopsis thaliana - terrestrial plants

(Received May 13, 2008; Accepted May 27, 2008)
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