Plant and Cell Physiology Advance Access published online on May 29, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn080
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RNAi-mediated knockdown of XIP-type endoxylanase inhibitor gene, OsXIP, has no effect on grain development and germination in rice.
Graduate School of Biosphere Sciences, Hiroshima University, 1-4-4, Kagamiyama, Higashi-Hiroshima, 739-8528 Japan
Corresponding author: Prof. Muneharu Esaka, Graduate School of Biosphere Sciences, Hiroshima University, 1-4-4, Kagamiyama, Higashi-Hiroshima, 739-8528 Japan. Telephone & Fax: 81-824-24-7927 (Extension: 7927), e-mail: mesaka{at}hiroshima-u.ac.jp
| Abstract |
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OsXIP (Oryza sativa xylanase inhibitor protein) is a XIP-type xylanase inhibitor which was identified as a protein encoded by a wound stress-responsive gene in rice. Although OsXIP gene was specifically expressed in mature grains under the basal condition, recombinant OsXIP had no effect on rice endogenous xylanases, and OsXIP suppressed transgenic rice plants did not exhibit any change in grain development and germination, suggesting that rice development may be independent of OsXIP. The analysis using OsXIP specific antibody revealed that OsXIP is markedly accumulated in apoplast in rice root cells by wound. These results reinforced the possibility that OsXIP is involved in plant defense mechanisms against phytopathogens.
Keywords: Oryza sativa - Xylanase inhibitor - Stress response
(Received March 29, 2008; Accepted May 25, 2008)
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