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Plant and Cell Physiology Advance Access published online on April 20, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn064
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Separation of Light-induced Linear, Cyclic and Stroma-sourced Electron Fluxes to P700+ in Cucumber Leaf Discs after Pre-illumination at a Chilling Temperature

Da-Yong Fan1,3, Alexander B. Hope2, Husen Jia3 and Wah Soon Chow3,*

1 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China
2 School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia
3 Photobioenergetics Group, Research School of Biological Sciences, Australian National University, Canberra, ACT 0200, Australia

*Corresponding author: Dr. Wah Soon Chow, Photobioenergetics Group, Research School of Biological Sciences, Australian National University Canberra, ACT 0200, Australia, Tel: +61 2 6125 3980, Fax: +61 2 6125 8056, E-mail: chow{at}rsbs.anu.edu.au


  Abstract

Pre-illumination of cucumber leaf discs at 4°C with low-irradiance white light (1) led to a marked decrease in the extent of photo-oxidation of P700 [the special chlorophyll pair in the photosystem I (PSI) reaction centre] in actinic light at room temperature and (2) hastened the post-illumination re-reduction of P700+. Quantifying the linear, cyclic and stroma-sourced electron fluxes to P700+ in two actinic-light regimes, we found that there was no increase in cyclic or linear electron fluxes to account for these changes. Rather, we observed a decrease in the maximum extent of P700 photo-oxidation assayed by a strong flash superimposed on continuous, background light of wavelength 723 nm, which we interpret to represent a loss of stable charge separation in PSI due to enhanced charge recombination as a result of the pre-illumination treatment. The funnelling of electrons towards fewer non-damaged PSI complexes could explain the hastened post-illumination re-reduction of P700+, aided by a slight increase in a stroma-sourced electron flux after prolonged pre-illumination at 4°C. Quantifying the separate fluxes to P700+ helps to elucidate the effects of chilling of cucumber leaf discs in the light and the reasons for the hastened post-illumination re-reduction of P700+.

Keywords: Cucumis sativus - cyclic electron flow - P700 - photoinactivation - photoinhibition - Photosystem

(Received January 7, 2008; Accepted April 15, 2008)
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