Skip Navigation



Plant and Cell Physiology Advance Access published online on April 15, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn061
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
49/6/865    most recent
pcn061v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ramamoorthy, R.
Right arrow Articles by Ramachandran, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramamoorthy, R.
Right arrow Articles by Ramachandran, S.
Agricola
Right arrow Articles by Ramamoorthy, R.
Right arrow Articles by Ramachandran, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

A Comprehensive Transcriptional Profiling of the WRKY Gene Family in Rice under Various Abiotic and Phytohormone Treatments

Rengasamy Ramamoorthy1, Shu-Ye Jiang1, Nadimuthu Kumar, Prasanna Nori Venkatesh and Srinivasan Ramachandran*

Rice Functional Genomics Group, Temasek Life Sciences Laboratory, 1 Research Link, the National University of Singapore, Singapore 117604

*Corresponding author: Dr. Srinivasan Ramachandran, Address: Rice Functional Genomics Group, Temasek Life Sciences Laboratory, 1 Research Link, the National University of Singapore, Singapore 117604. Telephone: 65-68727480, Fax: 65-68727007, E-mail: sri{at}tll.org.sg


  Abstract

WRKY transcription factors play important roles in the regulation of various biological processes. We have analyzed the rice genome sequence databases publicly available and predicted 103 genes encoding WRKY transcription factors. Among them, the majority of rice WRKY genes (77.7%) were located in duplicated regions; 45.6% of WRKY genes were fragmentally duplicated and 35% of them were tandemly duplicated. These results suggested that genome duplications might be regarded as a major mechanism for expansion of this family in rice genome. Subsequently, we analyzed their expression profiles under normal and abiotic stress as well as various hormone treatments. Under normal growth conditions, 65 WRKY genes were expressed differentially either in their transcript abundance or in their expression patterns. Under abiotic (cold, drought and salinity) stresses and various phytohormone treatments, 54 WRKY genes exhibited significant differences in their transcript abundance, among them 3 genes were expressed only in stressed conditions. Among the stress-inducible genes, 13 genes were regulated only by abiotic stresses, another set of 13 genes were responsive to only phytohormone treatments and the remaining 28 genes were regulated by both factors, suggesting an interaction between abiotic stress and hormone signaling. On the other hand, we have also surveyed expression divergence of duplicated genes under normal or stressed conditions and the results showed that high expression divergence has occurred not only among fragmentally but also among tandemly duplicated genes. These results suggested that the high expression divergence could be one of mechanisms for the retention of these duplicated WRKY genes.

Keywords: Expression profile - Oryza sativa cv Nipponbare - Real-time PCR

1 These authors contributed equally to this work

(Received January 28, 2008; Accepted April 9, 2008)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. P. Pandey and I. E. Somssich
The Role of WRKY Transcription Factors in Plant Immunity
Plant Physiology, August 1, 2009; 150(4): 1648 - 1655.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.