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Plant and Cell Physiology Advance Access published online on April 1, 2008

Plant and Cell Physiology, doi:10.1093/pcp/pcn053
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

ACTIN-RELATED RROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis

Muthugapatti K. Kandasamy, Elizabeth C. McKinney and Richard B. Meagher*

Genetics Department, Davison Life Sciences Building, University of Georgia, Athens, GA 30602

*Corresponding author: E-mail, meagher{at}uga.edu; Fax, 706 542-1387.


   Abstract

Arabidopsis encodes six nuclear actin related proteins (ARPs), among them ARP8 is unique in having a F-box domain and an actin-homology domain. Analysis of ARP8 promoter-GUS fusion suggests that ARP8 is ubiquitously expressed in all organs and cell types. Immunocytochemical analysis with ARP8-specific monoclonal antibodies revealed that ARP8 protein is localized to the nucleolus in interphase cells and dispersed in the cytoplasm in mitotic cells. The cell cycle-dependent subcellular patterns of distribution of ARP8 are conserved in other members of Brassicaceae. Our findings provide the first insight into the possible contributions of plant ARP8 to the nucleolar functions.

Keywords: Nuclear actin-related proteins - ARP8 - chromatin remodeling - ribosome biogenesis - nucleolar functions

(Received February 7, 2008; Accepted March 27, 2008)
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