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Plant and Cell Physiology Advance Access published online on February 28, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn036
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

DCW11, Down-regulated gene 11 in CW-type cytoplasmic male sterile (CMS) rice, encoding mitochondrial protein phosphatase 2C is related to CMS.

Sota Fujii and Kinya Toriyama

Affiliation and address: Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan

Corresponding author: Kinya Toriyama. Address: Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. Telephone number: +81-22-717-8830, Fax number: +81-22-717-8834, E-mail: torikin{at}bios.tohoku.ac.jp


  Abstract

Causes of cytoplasmic male sterility (CMS) in plants have been studied for two decades, and mitochondrial chimeric genes have been predicted to induce CMS. However, it is unclear what happens after CMS-associated proteins accumulate in mitochondria. In our previous study of microarray analysis, we found that 140 genes are aberrantly regulated in anthers of CW-type CMS of rice (Oryza sativa L.). In the present study, we investigated DCW11, one of the Down-regulated genes in CW-CMS encoding a protein phosphatase 2C (PP2C). DCW11 mRNA was preferentially expressed in anthers with the highest expression in mature pollen. As predicted by the N-terminal sequence, DCW11 signal peptide-GFP fusion protein was localized in mitochondria. Knockdown of DCW11 in wild-type rice by RNAi caused a major loss of seed-set fertility, without visible defect in pollen development. Since this knockdown phenotype resembled that of CW-CMS, we concluded that the down-regulation of DCW11 is correlated with CW-CMS. This idea was supported by the up-regulation of Alternative oxidase 1a (AOX1a), which is known to be regulated by mitochondrial retrograde signaling, in DCW11 knockdown lines. Down-regulation of DCW11 and up-regulation of AOX1a were also observed in two other types of rice CMS. Our result indicates that DCW11 could play a role in a mitochondrial signal transduction mediator in pollen germination.

Keywords: Oryza sativa - Cytoplasmic male sterility (CMS) - Protein phosphatase 2C - Mitochondrial retrograde signaling

(Received December 10, 2007; Accepted February 25, 2008)
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