Plant and Cell Physiology Advance Access published online on February 21, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn030
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The role of the electron transport in determining the temperature dependence of photosynthetic rate in spinach leaves grown at contrasting temperatures
1Department of Biology, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka, 560-0043, Japan
2Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
3Department of Life Science, University of Hyogo, 3-2-1 Kamigohri, Ako-Gun, Hyogo, 678-1297 Japan
Corresponding author, Wataru Yamori, Molecular Plant Physiology Group, Research School of Biological Sciences, Building 46, The Australian National University, Canberra, ACT, 2601 Australia, Tel: +61 2 6125 5430, Fax: +61 2 6125 5075, E-mail: wataru.yamori{at}anu.edu.au
 |
Abstract |
|---|
Temperature response of uncoupled whole-chain electron transport rate (ETR) in thylakoid membranes differs depending on the growth temperature. However, the steps that limit whole-chain ETR are still unclear and the question of whether the temperature dependence of whole-chain ETR reflects that of photosynthetic rate remains unresolved. Here, we determined the whole-chain, PS I and PS II ETR in thylakoid membranes isolated from spinach leaves grown at 30°C (HT) and 15°C (LT). We measured temperature dependencies of the light-saturated photosynthetic rate at 360 µL L-1 CO2 (A360) in HT and LT leaves. Both of the temperature dependences of whole-chain ETR and of A360 were different depending on the growth temperature. Whole-chain ETR was less than the rates of PSI ETR and PS II ETR in the broad temperature range, indicating that the process was limited by diffusion processes between the PS I and PS II. However, at high temperatures, whole-chain ETR appeared to be limited by not only the diffusion processes but also PS II ETR. The C3 photosynthesis model was used to evaluate the limitations of A360 by whole-chain ETR (Pr) and RuBP carboxylation (Pc). In HT leaves, A360 was co-limited by Pc and Pr at low temperatures, whereas at high temperatures, A360 was limited by Pc. On the other hand, in LT leaves, A360 was solely limited by Pc over the entire temperature range. The optimum temperature for A360 was determined by Pc in both HT and LT leaves. Thus, this study showed that, at low temperatures, the limiting step of A360 was different depending on the growth temperature, but was limited by Pc at high temperatures regardless of the growth temperatures.
Keywords: electron transport - photosynthesis - RuBP carboxylation - RuBP regeneration - temperature acclimation - temperature dependence
(Received December 31, 2007; Accepted February 18, 2008)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. I. Hozain, M. E. Salvucci, M. Fokar, and A. S. Holaday The differential response of photosynthesis to high temperature for a boreal and temperate Populus species relates to differences in Rubisco activation and Rubisco activase properties Tree Physiol, October 28, 2009; (2009) tpp091v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nagai and A. Makino Differences Between Rice and Wheat in Temperature Responses of Photosynthesis and Plant Growth Plant Cell Physiol., April 1, 2009; 50(4): 744 - 755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yamori, K. Noguchi, K. Hikosaka, and I. Terashima Cold-Tolerant Crop Species Have Greater Temperature Homeostasis of Leaf Respiration and Photosynthesis Than Cold-Sensitive Species Plant Cell Physiol., February 1, 2009; 50(2): 203 - 215. [Abstract] [Full Text] [PDF]
|
|