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Plant and Cell Physiology Advance Access published online on February 21, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn029
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Effects of conditional IPT-dependent cytokinin overproduction on root architecture of Arabidopsis seedlings

Alena Kuderová1, Ivana Urbánková1, Martina Válková1, Jirí Malbeck2, Danka Némethová3,4 and Jan Hejátko1

1. Laboratory of Plant Molecular Physiology, Department of Functional Genomics and Proteomics, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic
2. Institute of Experimental Botany, The Academy of Sciences of the Czech Republic, Rozvojová 135, 165 02 Prague, Czech Republic
3. Institute of Biostatistics and Analyses, Masaryk University, Kamenice 126/3, 625 00 Brno, Czech Republic
4. Research Centre for Environmental Chemistry and Ecotoxicology, Masaryk University, Kamenice 126/3, 625 00 Brno, Czech Republic

Corresponding author: Jan Hejátko, Department of Functional Genomics and Proteomics, Faculty of Science, Masaryk University, Kamenice 5, CZ-625 00, Brno, Czech Republic, phone # +420 5 4949 4165, fax # +420 5 4949 2640, e-mail hejatko{at}sci.muni.cz


  Abstract

Cytokinin (CK) has been known to inhibit primary root elongation and suggested to act as an auxin antagonist in the regulation of lateral root (LR) formation. While auxin role in root development has been thoroughly studied, the detailed and overall description of CK effects on root system morphology, particularly that of developing lateral root primordia (LRPs), and hence its role in organogenesis has still been in progress. Here we examine the effects of conditional endogenous CK overproduction on root architecture and consider its temporal aspect during the early development of Arabidopsis thaliana. We employed the pOp/LhGR system to induce ectopic ipt overexpression with a glucocorticoid dexamethason (DEX) at designated developmental points. The transient CaMV 35S>GR>ipt transactivation greatly enhanced levels of biologically active CKs of zeatin (Z)-type and identified a distinct developmental interval during which primary root elongation is susceptible to increases in endogenous CK production. Long-term CK overproduction inhibited primary root elongation by reducing quantitative parameters of primary root meristem, disturbed a characteristic graded distribution pattern of auxin response in LRPs and impaired their development. Our findings indicate impact of perturbed endogenous CK on the regulation of asymmetric auxin distribution during LRP development and imply that there is crosstalk between the auxin and cytokinin during organogenesis in A. thaliana.

Keywords: CaMV 35S>GR>ipt transactivation - endogenous cytokinin - DR5 activity - auxin-cytokinin crosstalk - root development - Arabidopsis thaliana

(Received December 1, 2007; Accepted February 18, 2008)
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