Skip Navigation



Plant and Cell Physiology Advance Access published online on February 23, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn023
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
49/3/457    most recent
pcn023v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Wenzel, C. L.
Right arrow Articles by Mattsson, J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wenzel, C. L.
Right arrow Articles by Mattsson, J.
Agricola
Right arrow Articles by Wenzel, C. L.
Right arrow Articles by Mattsson, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Identification of genes expressed in vascular tissues using NPA-induced vascular overgrowth in Arabidopsis

Carol L. Wenzel1, Qian Hester1 and Jim Mattsson1

1Department of Biological Sciences, Simon Fraser University, 8888 University Drive

Corresponding Author: Jim Mattsson. Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada, E-mail: mattsson{at}sfu.ca. Phone: 1-778-782-4291. Fax: 1-778-782-3496.


  Abstract

The genetic basis of vascular differentiation and function is relatively poorly understood, partly due to the difficulty of screening for mutants defective in internal vascular tissues. Here we present an approach based on a predicted increase in vascular-related gene expression in response to an auxin transport inhibitor-induced vascular overgrowth. We used microarray analyses to identify 336 genes that were up-regulated two-fold or more in shoot tissues of Arabidopsis thaliana showing vascular overgrowth. Promoter-marker gene fusions revealed that 38 out of 40 genes with four-fold or more up-regulation in vascular overgrowth tissues had vascular-related expression in transgenic Arabidopsis plants. Obtained expression patterns included cambial tissues and differentiating xylem, phloem and fibers. A total of 15 genes were found to have vascular-specific expression patterns in the leaves and/or inflorescence stems. This study provides empirical evidence of the efficiency of the approach and describes for the first time the in situ expression patterns of the majority of the assessed genes.

Keywords: auxin - vascular overgrowth - ascular expression

(Received December 3, 2007; Accepted February 1, 2008)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.