Skip Navigation



Plant and Cell Physiology Advance Access published online on January 25, 2008
Plant and Cell Physiology, doi:10.1093/pcp/pcn006
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
49/2/142    most recent
pcn006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Yamazaki, M.
Right arrow Articles by Hara-Nishimura, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamazaki, M.
Right arrow Articles by Hara-Nishimura, I.
Agricola
Right arrow Articles by Yamazaki, M.
Right arrow Articles by Hara-Nishimura, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Arabidopsis VPS35, a Retromer Component, Is Required for Vacuolar Protein Sorting and Involved in Plant Growth and Leaf Senescence

Misako Yamazaki1, Tomoo Shimada1, Hideyuki Takahashi1, Kentaro Tamura1, Maki Kondo2, Mikio Nishimura2 and Ikuko Hara-Nishimura1

1Graduate School of Science, Kyoto University, Kyoto, 606-8502 Japan
2Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444-8585 Japan

Corresponding author: Ikuko Hara-Nishimura, Graduate School of Science, Kyoto University, Kitashirakawa-oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan, Tel and Fax 81-75-753-4142, E-mail; ihnishi{at}gr.bot.kyoto-u.ac.jp


  Abstract

The retromer complex is responsible for retrograde transport, which is coordinated with anterograde transport in the secretory pathway including vacuolar protein sorting. Yeast VPS35 is a component of the retromer complex that is essential for recognition of specific cargo molecules. The physiological function of VPS35 has not been determined in vacuolar protein sorting in higher organism. Arabidopsis thaliana has three VPS35 homologs designated VPS35a, VPS35b and VPS35c. We isolated four vps35 mutants (vps35a-1, vps35b-1, vps35b-2 and vps35c-1) and then generated four double mutants and one triple mutant. vps35a-1 vps35c-1 exhibited no unusual phenotypes. On the other hand, vps35b-1 vps35c-1 and the triple mutant (vps35a-1 vps35b-2 vps35c-1) exhibited severe phenotypes: dwarfism, early leaf senescence and fragmentation of protein storage vacuoles (PSVs). In addition, these mutants mis-sorted storage proteins by secreting them out of the cells and accumulated a higher level of vacuolar sorting receptor VSR than wild type. VPS35 was localized in prevacuolar compartments (PVCs), some of which contained VSR. VPS35 was immunoprecipitated with VPS29/MAG1, another component of the retromer complex. Our findings suggest that VPS35, mainly VPS35b, is involved in sorting proteins to PSVs in seeds, possibly by recycling VSR from PVCs to Golgi complex, and is also involved in plant growth and senescence in vegetative organs.

Keywords: Arabidopsis - Protein trafficking - Retromer - Storage protein - VPS35 - VSR

(Received December 17, 2007; Accepted January 8, 2008)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
J. Wang, Y. Cai, Y. Miao, S. K. Lam, and L. Jiang
Wortmannin induces homotypic fusion of plant prevacuolar compartments
J. Exp. Bot., July 1, 2009; 60(11): 3075 - 3083.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Zouhar, E. Rojo, and D. C. Bassham
AtVPS45 Is a Positive Regulator of the SYP41/SYP61/VTI12 SNARE Complex Involved in Trafficking of Vacuolar Cargo
Plant Physiology, April 1, 2009; 149(4): 1668 - 1678.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
N. Q. Phan, S.-J. Kim, and D. C. Bassham
Overexpression of Arabidopsis Sorting Nexin AtSNX2b Inhibits Endocytic Trafficking to the Vacuole
Mol Plant, November 1, 2008; 1(6): 961 - 976.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. Hwang
Sorting and Anterograde Trafficking at the Golgi Apparatus
Plant Physiology, October 1, 2008; 148(2): 673 - 683.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Rojo and J. Denecke
What Is Moving in the Secretory Pathway of Plants?
Plant Physiology, August 1, 2008; 147(4): 1493 - 1503.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.