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Plant and Cell Physiology Advance Access published online on November 23, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm164
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Analysis of GDP-D-Mannose Pyrophosphorylase Gene Promoter from Acerola (Malpighia glabra) and Increase in Ascorbate Content of Transgenic Tobacco Expressing Acerola Gene

Adebanjo A. Badejo1, Nobukazu Tanaka2 and Muneharu Esaka1,*

1Graduate School of Biosphere Sciences, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan
2Center for Gene Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8527, Japan

Corresponding author: Dr. Muneharu Esaka, Graduate School of Biosphere Sciences, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Hiroshima, Japan. Tel: +81(0)82-424-7927, Fax: +81(0)82-424-7916, Email: mesaka{at}hiroshima-u.ac.jp


  Abstract

GDP-D-mannose pyrophosphorylase (GMP) is an important enzyme in the Smirnoff-Wheeler's pathway for the biosynthesis of ascorbic acid in plants. We have reported recently that the expression of acerola (Malpighia glabra) GMP gene designated MgGMP, correlates with the ascorbic acid content of the plant. The acerola plant has very high levels of ascorbic acid relative to better studied model plants such as Arabidopsis. Here we found that the GMP mRNA levels in acerola are higher than those from Arabidopsis and tomato. Also, the transient expression of the uidA reporter gene in the protoplasts of Nicotiana tabacum cultures showed MgGMP gene promoter with higher activity than those of cauliflower mosaic virus 35S and Arabidopsis GMP promoters. The ascorbic acid content of transgenic tobacco plants expressing MgGMP gene including its promoter were about 2-fold higher than those of the wild type.

Keywords: Acerola (Malpighia glabra) - Ascorbic acid - GDP-D-mannose pyrophosphorylase - Promoter analysis - Protoplast - Transgenic tobacco

(Received October 8, 2007; Accepted November 21, 2007)
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