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Plant and Cell Physiology Advance Access published online on November 23, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm162
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Tissue- and cell-specific localization of rice aquaporins and their water transport activities

Junko Sakurai1,2, Arifa Ahamed1, Mari Murai1, Masayoshi Maeshima3 and Matsuo Uemura2

1 Climate Change Research Team, National Agricultural Research Center for Tohoku Region, Morioka, 020-0198 Japan
2 United Graduate School of Agricultural Sciences and Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Morioka, 020-8550 Japan
3 Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan

Corresponding author: Junko Sakurai, National Agricultural Research Center for Tohoku Region, Morioka, 020-0198 Japan, Phone: (81) 19 643 3462, Fax: (81) 19 641 7794, E-mail: junkoi{at}affrc.go.jp


  Abstract

Water transport in plants is greatly dependent on the expression and activity of water transport channels, called aquaporins. Here, we have clarified tissue- and cell-specific localization of aquaporins in rice plants by immunoblotting and immunocytochemistry using seven isoform-specific aquaporin antibodies. We also examined water transport activities of typical aquaporin family members using a yeast expression system in combination with a stopped-flow spectrophotometry assay. OsPIP1 members, OsPIP2;1, OsTIP1;1 and OsTIP2;2 were expressed both in leaf blades and roots, while OsPIP2;3, OsPIP2;5 and OsTIP2;1 were expressed only in roots. In roots, aquaporins accumulated highly in the region adjacent to the root tip (around 1.5 to 4 mm from the root tip). In this region, cell-specific localization was observed with the various aquaporin members. OsPIP1 members and OsTIP2;2 accumulated predominantly in endodermis and the central cylinder, respectively. OsTIP1;1 showed specific localization in rhizodermis and exodermis. OsPIP2;1, OsPIP2;3 and OsPIP2;5 accumulated in all root cells, but they showed higher accumulation in endodermis than other cells. In the region at 35 mm from the root tip, where aerenchyma develops, aquaporins accumulated at low levels. In leaf blades, OsPIP1 members and OsPIP2;1 were localized mainly in mesophyll cells. OsPIP2;1, OsPIP2;3, OsPIP2;5 and OsTIP2;2 expressed in yeast showed high water transport activities. These results suggest that rice aquaporins with various water transport activities may play distinct roles in facilitating water flux and maintaining the water potential in different tissues and cells.

Keywords: Aquaporin - Cell-specific localization - Rice - Water transport activity

(Received August 30, 2007; Accepted November 16, 2007)
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