Skip Navigation



Plant and Cell Physiology Advance Access published online on November 14, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm157
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
49/1/117    most recent
pcm157v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ohnuma, M.
Right arrow Articles by Tanaka, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohnuma, M.
Right arrow Articles by Tanaka, K.
Agricola
Right arrow Articles by Ohnuma, M.
Right arrow Articles by Tanaka, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Polyethylene glycol (PEG)-mediated transient gene expression in a red alga, Cyanidioschyzon merolae 10D

Mio Ohnuma1, Takashi Yokoyama2, Takayuki Inouye2, Yasuhiko Sekine2 and Kan Tanaka1

1Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
2Department of Life Science, College of Science, Rikkyo (St. Paul’s) University, Nishiikebukuro, Tokyo, 171-8501 Japan

Corresponding author: Kan Tanaka. 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan, TEL: 03-5841-7826, FAX: 03-5841-8476, E-mail: kntanaka{at}iam.u-tokyo.ac.jp


  Abstract

DNA introduction into cells is an essential technique for molecular genetic analysis. Here, we show that DNA is easily introduced into cells of the unicellular red alga Cyanidioschyzon merolae by a PEG-mediated protocol. In this study, the ß-tubulin gene of C. merolae was cloned on a plasmid and a hemagglutinin (HA)-tag then added at the carboxy-terminus. This plasmid was then introduced into C. merolae cells by a PEG-mediated transformation protocol. 24 hours after PEG-mediated transformation, intracellular localization of the tagged protein was detected by anti-HA immunocytochemistry, indicating the utility of this transient expression system for molecular genetic analyses.

Keywords: Cyanidioschyzon merolae - hemagglutinin - polyethylene glycol - transformation - transient expression system

(Received September 21, 2007; Accepted November 8, 2007)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Imamura, Y. Kanesaki, M. Ohnuma, T. Inouye, Y. Sekine, T. Fujiwara, T. Kuroiwa, and K. Tanaka
R2R3-type MYB transcription factor, CmMYB1, is a central nitrogen assimilation regulator in Cyanidioschyzon merolae
PNAS, July 28, 2009; 106(30): 12548 - 12553.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.