Skip Navigation



Plant and Cell Physiology Advance Access published online on October 30, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm139
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
48/11/1524    most recent
pcm139v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ukitsu, H.
Right arrow Articles by Shinozaki, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ukitsu, H.
Right arrow Articles by Shinozaki, K.
Agricola
Right arrow Articles by Ukitsu, H.
Right arrow Articles by Shinozaki, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Cytological and Biochemical Analysis of COF1, an Arabidopsis Mutant of an ABC Transporter Gene

Hiroki Ukitsu1,2,5, Takashi Kuromori2,5, Kiminori Toyooka2, Yumi Goto2, Ken Matsuoka2,6, Eiji Sakuradani3, Sakayu Shimizu3, Asako Kamiya2, Yuko Imura2, Masahiro Yuguchi2, Takuji Wada2, Takashi Hirayama1,4 and Kazuo Shinozaki2,*

1 International Graduate School of Arts and Science, Yokohama City University, Yokohama, Kanagawa, 230-0045 Japan
2 Gene Discovery Research Group, RIKEN Plant Science Center, Yokohama, Kanagawa, 230-0045 Japan
3 Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, 606-8502 Japan
4 Environmental Molecular Biology Laboratory, RIKEN Discovery Research Institute, Wako, Saitama, 351-0198 Japan

*Corresponding author: Dr. Kazuo Shinozaki, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan, TEL: +81-45-5039579, FAX: +81-45-5039580, e-mail: sinozaki{at}rtc.riken.jp


  Abstract

In transposon-tagged lines of Arabidopsis we found two new mutants, cof1-1 and cof1-2 (cuticular defect and organ fusion), that show the phenotype of wilting when grown in soil, organ fusion of rosette leaves, and infertility. Toluidine-blue testing and scanning electron microscopy observation revealed that these mutants had cuticular defects in the stems and adult leaves, but not in cotyledones. Transmission electron microscopy observation revealed thinner cuticle layers in the mutants, and cuticular materials interspersed between the two fused epidermal layers were observed in the mutant rosette leaves. These two mutants had a transposon insertion in the coding regions of WBC11, which was classified as a member of ABC transporter genes in Arabidopsis. WBC11 showed high sequence similarity to CER5 (also called WBC12), which was involved in cuticular lipid export. Gas-chromatographic analysis revealed that C29 alkane extracted from the stem surface of cof1 mutants was reduced whereas C29 ketone was accumulated, which was different from the case of cer5 mutants. While cer5 mutants had fairly normal morphology, cof1 mutants had pleiotropic phenotypes so that the COF1/WBC11 could have other important roles than CER5/WBC12. We also found that C29 alkane was accumulated in the intracellular extraction of cof1 mutants, suggesting thefunction of WBC11 in the direct transport of lipid molecules. Pollen observation showed mutant pollen grains were irregularly shaped. The function of COF1/WBC11 in lipid transport for the construction of cuticle layers and pollen coats for normal organ formation will be discussed.

Keywords: ABC transporter - Arabidopsis thaliana - cuticle - organ fusion - transposon-tagged line

5These authors contributed equally to this work.

6Present address: Faculty of Agriculture, Kyusyu University, Fukuoka, Fukuoka, 812-8581 Japan.

(Received September 30, 2007; Accepted October 10, 2007)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. Mintz-Oron, T. Mandel, I. Rogachev, L. Feldberg, O. Lotan, M. Yativ, Z. Wang, R. Jetter, I. Venger, A. Adato, et al.
Gene Expression and Metabolism in Tomato Fruit Surface Tissues
Plant Physiology, June 1, 2008; 147(2): 823 - 851.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.