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Plant and Cell Physiology Advance Access published online on September 12, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm117
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Contribution of anaphase B to chromosome separation in higher plant cells estimated by image processing

Tomomi Hayashi1,3,*, Toshio Sano1,3, Natsumaro Kutsuna1, Fum Kumagai-Sano2 and Seiichiro Hasezawa1

1Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba, 277-8562 Japan
2Department of Science Education, Faculty of Education, Gunma University, Aramaki-cho 4-2, Maebashi, Gunma, 371-8510 Japan

Corresponding author, Tomomi Hayashi. FSB701, The University of Tokyo, 5-1-5, Kashiwanoha, Kasiwa, 277-8562 Japan, TEL: +81-4-7136-3708, FAX: +81-4-7136-3706, E-mail: kk077514{at}mail.ecc.u-tokyo.ac.jp


  Abstract

Anaphase can be categorized into the two sub-phases of anaphase A and B, but anaphase B has not been clearly described in higher plant cells. In this study, we time-sequentially followed the dynamics of chromosome segregation and spindle elongation in tobacco BY-2 cells using histone-RFP and GFP-tubulin, respectively. Construction of kymographs and determination of the positions of chromosomes and spindle edges by image processing revealed that anaphase B contributed to about 40 % of the chromosome separation in distance, which is comparable to that in animal cells. These results suggest that higher plant cells potentially possess the process of anaphase B.

Keywords: Anaphase - Fluorescent proteins - Image processing.

3These authors contributed equally to this work.


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