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Plant and Cell Physiology Advance Access published online on August 24, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm114
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Isolation of SIP, a protein that interacts with SPB, a possible transcriptional regulatory factor in Rhodobacter sphaeroides

Hiroshi Shimada1, Kazuhiro Ishida1, Yasuhiro Machiya and Ken-ichiro Takamiya2

Corresponding author: Hiroshi Shimada. Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259, Nagatsuta-cho, Midori-ku, Yokohama, Japan. Telephone: +81-45-924-5737, Fax: +81-45-924-5823, E-mail: hshimada{at}bio.titech.ac.jp


  Abstract

SPB is a transcriptional factor in Rhodobacter sphaeroides that represses expression of the puf operon under aerobic or semi-aerobic light conditions. Here, we identified a 17,500 protein designated SIP (SPB Interaction Protein) that interacts with SPB, as determined by binding to an SPB-Hisx6 fusion protein-Ni column. The SPB-SIP interaction in vivo was confirmed by an immunoprecipitation assay. The level of transcripts and protein of SIP did not differ for all growth conditions tested, indicating that regulation of the SIP-SPB interaction, if any, is not through modulation of sip or spb expression but rather by modification of the proteins

Keywords: H-NS-like protein - protein-protein interaction - Rhodobacter sphaeroides - SIP - SPB

1These authors contributed equally to the work.

2Professor Ken-ichiro Takamiya passed away during the course of this study following an unfortunate traffic accident.


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