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Plant and Cell Physiology Advance Access published online on August 22, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm106
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Cyanobacteriochrome TePixJ of Thermosynechococcus elongatus harbors phycoviolobilin as a chromophore.

Takami Ishizuka1, Rei Narikawa1, Takayuki Kohchi2, Mitsunori Katayama1 and Masahiko Ikeuchi1

1Department of Life Sciences (Biology), The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan.
2Graduate School of Biostudies, Kyoto University, Kitashirakawa-oiwake, Sakyo, Kyoto 606-8502, Japan.

Corresponding author: Masahiko Ikeuchi. Mailing address: Department of Life Sciences (Biology), The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan. Phone: 81-3-5454-6641. Fax: 81-3-5454-4337. E-mail: mikeuchi{at}bio.c.u-tokyo.ac.jp


  Abstract

Cyanobacteria have several putative photoreceptors (designated cyanobacteriochromes) that are related to but distinct from the established phytochromes. The GAF domain of the phototaxis regulator, PixJ, from a thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 (TePixJ_GAF) is a cyanobacteriochrome which exhibits reversible photoconversion between a blue light-absorbing form (max = 433 nm) and a green light-absorbing form (max = 531 nm). To study the chromophore, we prepared TePixJ_GAF chromoprotein from heterologously expressed Synechocystis and performed spectral analysis after denaturation by comparing with the cyanobacterial phytochrome Cph1 which harbors phycocyanobilin (PCB) as a chromophore. Results indicated that the chromophore of TePixJ is not PCB, but an isomer is phycoviolobilin (PVB). It is suggested that the GAF domain of TePixJ has auto-lyase and auto-isomerase activities.

Keywords: auto-isomerase - cyanobacteria - cyanobacteriochrome - phycocyanobilin - phycoviolobilin - phytochrome


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