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Plant and Cell Physiology Advance Access published online on August 3, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm099
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Location of possible miRNA processing site in SmD3/SmB nuclear bodies in Arabidopsis

Yoichiro Fujioka1, Maki Utsumi1, Yusuke Ohba2,1 and Yuichiro Watanabe1,*

1 Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, JAPAN
2 Department of Laboratory Medicine, Hokkaido University Graduate School of Medicine, N15 W7, Kita-ku, Sapporo 060-8638, JAPAN.

*Corresponding author: Yuichiro Watanabe, Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan, Tel/Fax 81-3-5454-6776, e-mail: solan{at}bio.c.u-tokyo.ac.jp


  Abstract

There has been much recent research on the contribution of microRNA (miRNA) in plant organogenesis and hormone action. In plants, it has been reported that Dicer-like 1 (DCL1), HYPONASTIC LEAVES1 (HYL1) and SERRATE (SE) are involved in the production of miRNAs. The means by which miRNAs are processed and transported is not well understood in detail, however. In this study we investigated the intracellular localization and intermolecular interaction of these molecules using imaging techniques, including bimolecular fluorescence complementation and fluorescence resonance energy transfer techniques, making use of various enhanced fluorescent proteins. We found that DCL1, HYL1 and SE formed bodies which localized in the nuclei. We were also able to locate the miRNA primary transcript using an MS2-tagged method on the bodies. It appears very likely that the observed DCL1-HYL1-SE body is involved in miRNA production. Co-expression of SmD3 or SmB proteins revealed the localization of DCL1-HYL1-SE complexes in the SmD3/SmB nuclear bodies.

Keywords: Arabidopsis - Dicer-like 1 - HYL1 - micro RNA - processing - SERRATE


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