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Plant and Cell Physiology Advance Access published online on July 29, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm097
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts

Hiroshi Kuroda1, Haruka Suzuki1, Takahiro Kusumegi2,4, Tetsuro Hirose2,5, Yasushi Yukawa1 and Masahiro Sugiura1,2,3,*

1Graduate School of Natural Sciences, Nagoya City University, Yamanohata, Mizuho, Nagoya 467-8501, Japan
2Center for Gene Research, Nagoya University, Nagoya 464-8602, Japan
3Sugiyama Human Research Center, Sugiyama Jogakuen University, Nagoya 464-8662, Japan

Corresponding author: Masahiro Sugiura. Graduate School of Natural Sciences, Nagoya City University, Yamanohata, Mizuho, Nagoya 467-8501, Japan, Phone: +81-52-781-7137 Fax: +81-52-781-7196 E-mail: sugiura{at}nsc.nagoya-cu.ac.jp


  Abstract

Plastid psbC gene encodes a CP43 subunit of Photosystem II. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions were generally annotated from the upstream AUG. Using an chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno(SD)-like sequence, GAGGAGGU, 9-nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3'-end of 16S rRNA facilitates translation initiation from the GUG.

Keywords: In vitro - psbC - Shine-Dalgarno sequence - Tobacco - Translation

4Present address: Toyota Motor Corporation, Toyota, Toyota 471-8572, Japan

5Present address: National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064, Japan


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