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Plant and Cell Physiology Advance Access published online on July 10, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm090
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

The jasmonate-induced expression of the Nicotiana tabacum leaf lectin

Nausicaä Lannoo1, Gianni Vandenborre1,2, Otto Miersch3, Guy Smagghe2, Claus Wasternack3, Willy J. Peumans1 and Els J.M. Van Damme1,*

1Laboratory of Biochemistry and Glycobiology, Dept. of Molecular Biotechnology, Ghent University, Coupure Links 653, 9000 Gent, Belgium
2Laboratory of Agrozoology, Dept. of Crop Protection, Ghent University, Coupure Links 653, 9000 Gent, Belgium
3Department of Natural Product Biotechnology, Leibniz Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle/Saale, Germany

*Corresponding author: Els J.M. Van Damme, Department of Molecular Biotechnology, Lab. Biochemistry and Glycobiology, Ghent University, Coupure Links 653, B-9000 Gent, Belgium, E-mail: ElsJM.VanDamme{at}UGent.be, Fax +32 92646219, Tel. +32 92646086


  Abstract

Previous experiments with tobacco (Nicotiana tabacum L. cv Samsun NN) plants revealed that jasmonic acid methyl ester (JAME) induces the expression of a cytoplasmic/nuclear lectin in leaf cells and provided the first evidence that jasmonates affect the expression of carbohydrate-binding proteins in plant cells. To corroborate the induced accumulation of relatively large amounts of a cytoplasmic/nuclear lectin a detailed study was performed on the induction of the lectin in both intact tobacco plants and excised leaves. Experiments with different stress factors demonstrated that the lectin is exclusively induced by exogeneously applied jasmonic acid and JAME, and to a lesser extent by insect herbivory. The lectin concentration depends on leaf age and the position of the tissue in the leaf. JAME acts systemically in intact plants but very locally in excised leaves. Kinetic analyses indicated that the lectin is synthesized within 12 hours exposure time to JAME, reaching a maximum after 60 hours. After removal of JAME, the lectin progressively disappears from the leaf tissue. The JAME induced accumulation of an abundant nuclear/cytoplasmic lectin is discussed in view of the possible role of this lectin in the plant.

Keywords: inducible protein - jasmonate - lectin - Nicotiana tabacum - Spodoptera littoralis - tobacco


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