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Plant and Cell Physiology Advance Access published online on June 27, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm080
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Transactivation of Protein Expression by Rice HSP101 in planta and Using Hsp101 as a Selection Marker for Transformation

Ching-Chun Chang1,*, Pin-Sheng Huang1, Huei-Ru Lin1 and Chun-Hsien Lu1

1. Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan 701

*Corresponding author: Ching-Chun Chang. To whom correspondence should be addressed. Tel: +886-6-2757575 ext 65623; E-mail: chingcc{at}mail.ncku.edu.tw


  Abstract

Plant HSP101 has dual activities, first, in conferring thermotolerance, second, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacteria-mediated transformation. Stable integration and expression of the transgene into the tobacco genome was demonstrated by Southern and western blot analysis. Overexpression of osHSP101 had no noticeable effect on growth or development of the transgenic plants. Homozygous T2 transgenic plants with overexpressed osHSP101 survived heat treatment better than untransformed control plants. In addition, taking advantage of conferring basal thermotolerance by plant HSP101, we were able to demonstrate the feasibility of using osHsp101 as a selection marker and select the transformants under high temperature in tobacco leaf disc transformation mediated by Agrobacteria. Furthermore, transgenic tobaccos with overexpressed osHSP101 were able to enhance luciferase expression up to 2.9 fold higher than untransformed plants in the progenies of reciprocally crossed with omega-luciferase reporter lines.

Keywords: HSP101 - thermotolerance - transgenic tobacco - selection marker - translational activator


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