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Plant and Cell Physiology Advance Access published online on June 6, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm069
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Differential expression of alternatively spliced mRNAs of Arabidopsis SR protein homologues, atSR30 and atSR45a, in response to environmental stress

Noriaki Tanabe1, Kazuya Yoshimura2, Ayako Kimura1, Yukinori Yabuta1 and Shigeru Shigeoka1,*

1Advanced Bioscience, Graduate School, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan
2Department of Food and Nutritional Science, Collage of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan

Corresponding author: Shigeru Shigeoka. Advanced Bioscience, Graduate School, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan. Tel: +81-742-43-8083, FAX: +81-742-43-8976, E-mail: shigeoka{at}nara.kindai.ac.jp


  Abstract

Serine/arginine-rich (SR) proteins are associated with either the regulation or the execution of both the constitutive splicing and the selection of alternative splice sites in animals and plants. We demonstrated the molecular characterization of a homologue of SR protein, atSR45a, in Arabidopsis plants. Six types of mRNA variants (atSR45a-1a ~ e and atSR45a-2) were generated by the alternative selection of transcriptional initiation sites and the alternative splicing of introns in atSR45a pre-mRNA. The atSR45a-1a and -2 proteins, presumed mature forms, were located in the nucleus and interacted with the U1-70K, suggesting that these proteins function as a splicing factor in Arabidopsis. The transcript levels of atSR45a and atSR30, SF2/ASF-like SR protein, were increased by various types of stress, such as high-light irradiation and salinity. Furthermore, splicing patterns of atSR45a and atSR30 pre-mRNA themselves were altered under these stressful conditions. In particular, the expression of atSR45a-1a, atSR45a-2, atSR30 mRNA1, and atSR30 mRNA3 was highly increased by high-light irradiation. These results indicate that the regulation of transcription and alternative splicing of atSR45a and atSR30 are responsive to various stressful conditions.

Keywords: SR protein - Alternative splicing - Arabidopsis - high-light irradiation


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